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We narrowed to 957 results for: REP

Showing: 521 - 540 of 957 results

  1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop at TAGC with the three...engaged through a computer screen. For the Reproducibility for Everyone Workshop, Abitua and the other...knowing you're suppose to draw Spongebob? The Reproducibility for Everyone Workshop included this interactive... activity during their session. Image from Reproducibility for Everyone.  While this type of interaction...

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...species of bacteria. The stain colored one type, Streptococcus pneumoniae, while the alcohol used in the staining...Klebsiella pneumoniae. After realizing this was a repeatable phenomenon, Gram began using it as a classification... positive bacteria include staphylococci and streptococci. Gram negative bacteria, or diderms, have a ...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions...

  3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation for therapeutic...reflect how cancer/immunology researchers are preparing and carrying out this multi-pronged attack. Inflammation...This immunosuppressive environment in turn may represent a favorable state for tumor progression (Ref 2...potential to become an open-source pipeline and data repository for cancer researchers. Go open-source! Impressions...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos... reverse the orientation of the crRNA’s direct repeats when expressing the Cpf1 multiplex system from ...virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1...This undoes the original reversing of the direct repeats, leading to expression of a crRNA array that can...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...mentoring can work if both parties are diligent about replying and thinking about good questions and answers....Thank them anyway and move on. If they say yes, be prepared with questions and topics to discuss. The mentee...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When expressed...multiple cloning site (MCS) into the α-peptide (represented as an orange wedge in the figure on the left)...-complementation (cell B). As shown on the representative plate to the right, colonies with an insert-containing...defined medium, without which the cells cannot reproduce to form colonies (used to complement auxotrophic...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...The transgene sequence is flanked by long terminal repeat (LTR) sequences, which facilitate integration of... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3-R-U5 regions found on either side of a retroviral...activation of viral genomic RNA transcription. R; Repeat region found within both the 5' and 3' LTRs of ...

  8. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...spend more prep time on an experiment than it is to rerun an experiment, so consider your prep an investment... used to constantly underestimate time needed to prep for an experiment (labeling tubes never takes longer...harder to start earlier, but simply moving all my prep work to the day before, which got rid of the issue...

  9. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this...monoclonal antibodies, they also lead to more reproducible experimental results. When you are originally...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...thing’ could be a neurotransmitter, such as norepinephrine, or an electrolyte such as calcium. This varied...transducer will generate a numerical value which represents the amount of analyte present in the system at...These sensors have been tested in vivo, in tissue preps, in multiple species, and in cell culture models... several years!). Biosensors tend to be more reproducible when used as stable systems, rather than transiently...

  11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...manually transforms, innoculates, and performs DNA minipreps for small batches of samples. As soon as that ...that process is complete, QC scientists prepare Sanger reactions by mixing this isolated plasmid DNA with... each group of plates will have select samples prepared and screened by NGS to spot check the samples,...

  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... bioinformatic screens that looked for a CRISPR repeat array near a putative effector nuclease. The Cas13d...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing from ...Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional Activators...

  13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...break in the genome anywhere you would like. Cells repair those breaks in one of two ways: by putting the... a gene. One can also exploit homology-directed repair by flooding the cell with a mutant template and...and fooling the cell into using that template for repair. By using these two scenarios, you can either knock...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  14. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...conditions, i.e. with low excitation power. By repeating these measurements for different fluorescent proteins... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...a set-up has been changed (for instance after replacing a broken lightbulb or exchanging excitation filters...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...How do microenvironment enzymes and metabolic reprograming influence tumors development? Being pathological...cells improve tumor’s durability, while others repress it. Unfortunately, as a whole, microenvironments...microenvironments promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand...affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential for a...

  17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for... standardized parts, it allows for greater reproducibility as scientists can assemble parts from different...sequences for genomic integration by homology directed repair. Using parts from this kit, the McCormick lab generated...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC -3’, where NNN and XXX represent the 20-bp gRNA target sequence and its 8-bp barcode...and applications in functional genomics, cell reprogramming, and beyond. For further details, please check..."Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 (2014): 647-661. ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

  19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...order to head to Ireland and take a chance as entrepreneurs. To make things more interesting, we are working...career, but it was enough to start us down the entrepreneurial path. We threw some of our own money at this...genetically modified organisms, and GMOs have a bad reputation. The public is can be fearful of this technology...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...

  20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...assertive communication.” - Jim Rohn, Author, Entrepreneur, Motivational Speaker Do you have a difficult...supervisor might expect weekly or biweekly progress reports. However, a hands-off busy supervisor might get...update, and whether he or she would like a written report or to meet in person.   Regardless of your supervisor...for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are busy...
Showing: 521 - 540 of 957 results