We narrowed to 559 results for: SEC

...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better...

...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...

... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...

...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...

...Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered... when purifying the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 ...

...of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3...

...Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial membrane...

... flow cytometry. Let's briefly go through each section of this article again. In the beginning, we talked...

...fact is that we are citizens first and scientists second and that it is our duty to tell our science stories...

...can transfer energy directly via resonance to the second fluorophore (the acceptor) if the two probes are...

...kit.   Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking...

.... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

...first gRNA using restriction enzyme BbsI and the second gRNA using restriction enzyme BsaI. If you’re working...

...add 10 µL of the pool to 990 µL LB then perform a second and then third 1:100 dilution). Plate 100 µL of...not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12-18 ...

...Higher efficiency ✓ ✓ ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand ...

...vectors are better suited for these situations. The second scenario requires higher levels of safety and as...

...design is not as efficient in genome editing as the second design (in pX330/PX335), so we recommend people...

...in this way. As described in the above CRISPRi section, the Marraffini Lab published a robust tool for...

...telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted. Reduction in...

...digestion with EcoRI and AgeI. The oligos from section B contain the shRNA sequence flanked by sequences...polymerase has difficulty reading through the secondary structure of the hairpin sequence. Back to Top...