Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 672 results for: abo.1

Showing: 521 - 540 of 672 results

  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

  3. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...faster. Years of science, done in weeks Figure 1: Sending out 80 FedEx envelopes in a single day --...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...are particularly interested in facilitating collaborative science, and making it accessible to all people...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    ...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

  5. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...present in the data such as synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture multiple...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

  6. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...

  7. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  8. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image used...clarifying roles, I eased the tension and improved collaboration. The good news: It’s a journey, not a final...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...into account, the digest pattern depicted in Lane 1 would be expected for a XbaI/NotI digest. The program...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated...diluting - if you end up concentrating your AAV above your desired titer and want to dilute it, err on...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR ...

  12. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    ...as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head ... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

  14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

  15. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

  16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... Effectors.” Cell (2018) pii: S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper ...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...this paper are available at Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources... Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find...

  17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...speed: base editing before Cas lets go Figure 1: David Liu gives a talk at the Cell Symposia: Gene...posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series...both changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased...change C to T, G to A, A to G, and T to C, represent about two-thirds of known pathogenic point mutations. ...

  18. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...the output of the analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word...the name, date, and location of all analyses (see above). Give every experiment its own directory: In ...record your goal, approach, and conclusions (see above). Keep original files in a separate folder: You... can simply make a note in your methods section about the edit that was temporarily used. Just be sure... Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time and Track Your...

  19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

  20. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...prove the technology. This transition takes around 1 – 2 years. The product is the measure of success...transitioned away from the bench and became all about finding the best product-market fit for this set...Notably, the team must have an upfront conversation about its goals and vision for the company; this will ...section! And read on for more great information about starting a biotech company. Suggested reading The...of Sample6) for taking the time to speak with us about the biotech startup process and work environment...
Showing: 521 - 540 of 672 results