We narrowed to 821 results for: eed

...system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

... pose the greatest threat, cancerous cells still need to successfully infiltrate the circulatory or lymph...include several other important pathways. We invite feedback and contributions from all members of the research...

...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...

...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ...

...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...

...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

...is the point?  Antibodies are large proteins that need a little help crossing cell membranes as they are...the inside of the cell. However, assuming you do need to permeabilize, you have a few options for what...

...between the size of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the...protein. In some cases this property eliminates the need for removing excess dye from the sample, which is...

...world, coordinates alone aren’t very useful. We’ll need something like Google Maps to help us identify and...NANOG enhancers from step 1 with genome math, we’ll need a bit of shorthand from the field of epigenomics...

...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide...

...immediately transformed and stored. Some samples need to be transformed with a micropulser using an electric...samples require a reduced antibiotic concentration or need to be transformed into different cells than were...

... adjacent motif) near the target DNA, TnpBs also need a short sequence motif called a TAM (transposon-... used to generate gene-edited plants without the need for tissue culture (Maher et al., 2020; Qiao et ...

...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

... neuronal synapses for instance. Also these tags need to be fused to scaffold proteins in order to be ...composed of FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically...

...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher...

...in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite...understand where sonic hedgehog got its name, we need to go back to 1980, when Christine Nusslein-Volhard...