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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 521 - 540 of 830 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  10. MXS Chaining

    Type
    Blog Post
    ...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide...

  16. CRISPR Guide

    Type
    Guide
    ...requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to the gene of ...Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting...) will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...NHEJ directly ligates the break ends without the need for a homologous template. Both NHEJ and HDR have... the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...matches that of the original library. Next, you’ll need to generate lentivirus containing the entire library...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at the end...table . In order to produce lentiviral vectors, you need three (or four, for third generation) plasmids: ...heterologous promoter (often CMV or RSV), eliminating the need for transactivation by Tat. Most third-generation...Biology Reference . Production After cloning, you will need to produce the lentiviral vectors from your plasmids...with contact to mucous membranes or broken skin. Needle sticks and ripped gloves are common points of entry...definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus. Third-generation...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015...
Showing: 521 - 540 of 830 results