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...Finally a set of wild type and codon optimized, full length and truncated versions of SARS-CoV-2 S (spike protein...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput...

...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...

...the utilization and development of genetically engineered components, like protein tags (Figure 2). Through...Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection Promega...

...SciComm by doing SciComm Writing had always been a strength of Susan’s, but she didn’t know if she liked science...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some...

...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing...

...time. Searching for literature My go-to search engines for finding scientific literature are Google Scholar...relevant. Harness the power of the Google search engine and sign up for Google Scholar alerts. Based on...

...Therefore they make excellent tools for genetic engineering. There are several different viral vectors to...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...

...and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs...

...decaffeination operon podcast segment With the help of engineered microbes, used coffee grounds could be economically...generalized to measure phosphatase activity, second messenger activity, and protein-protein interactions. We...

...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...

... Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits...Gateway cloning. In addition, scientists have engineered entirely new types of plasmids without clear ...

...Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime editors...

... time out of their day to talk with you, so be engaged with their conversation. Send thank you emails ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

...safety features   When viruses were first being engineered as biological tools, thought was given on how...replication of virus and its infectivity in check. Engineering controls, safety systems that are built into ...

...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...Researchers in the Gradinaru lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac...

...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...

...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...