Mammalian RNAi Tools
Type
Collection
...the viral vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an ...
We narrowed to 539 results for: genome
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CRISPR 101: Cytosine Transversion Editors
TypeBlog Post..., & Chew, W. L. (2021). Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision... -
Molecular Biology Reference
TypeGuide... of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome editing is most ...thousand basepairs from the entire human genome. Digesting the human genome with restriction enzymes would yield...plasmids upon cell division in addition to their own genomic DNA. Because of this additional replication burden...guide RNAs that target specific locations in the genome. For more information on CRISPR check out Addgene...Viral Plasmids - These plasmids are modified viral genomes that are used to efficiently deliver genetic material... to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would give you ... -
AAV for Neuronal Tracing
TypeCollection...required for the transcription or replication of the genome within infected cells, but is required for transynnaptic... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...Chen et al., 2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid... screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 and thus produces... -
Antibody Plasmid Collection
TypeCollection...of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822... -
Science Guides
TypeGuide...immune system in bacteria, have been modified for genome engineering. Find essential background information...genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets... -
Validated gRNA Sequences
TypeCollection...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce... Genome Engineering CRISPR Validated gRNA Sequence Datatable... -
Open Enzyme Collection
TypeCollection...in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology,... -
Using AAV for Neuronal Tracing
TypeBlog Post...introduce genetic material into neuronal cells. The AAV genome is a single-stranded DNA molecule and AAV vectors... -
Educational Resources
TypeGuide...related molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors... -
Addgene's eBook Collection
TypeGuide...designing your gRNA and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent... -
Neurodegeneration Research Collection
TypeCollection...associated with Alzheimer's disease, through large genome-wide association studies (GWAS) or other large ... -
COVID-19 Resources
TypeCollection... Image credit: Maya Kostman for the Innovative Genomics Institute The Coronavirus Disease-2019 (COVID-... -
Luciferase Plasmid Collection
TypeCollection... (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers using a firefly luciferase... -
Cloning
TypeGuide... like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO... -
Genetic Code Expansion
TypeCollection...69493 MCJ.559 TAG sites, RF1 function removed, genomic deletions for improved incorporation Michael Jewett... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...Ronda et al., 2019). They called their method Metagenomic Alteration of Gut microbiome by In situ Conjugation... PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in... -
dTAG - You're it!
TypeBlog Post...Erb et al., Huang et al.), transcriptional and epigenomic profiling studies (Nabet et al., Erb et al., ...