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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 568 results for: mme

Showing: 521 - 540 of 568 results

  3. Typing CRISPR Systems

    Type
    Blog Post
    ... C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...arising from the secondary antibody.  It is also recommended that users include a viability control as this...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiency of the virus, so Addgene recommends that you use the virus immediately or aliquot the media into smaller...pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing oligos C.3 Digesting ...transforming into bacteria D. Screening for Inserts D.1 Recommended materials D.2 Screening for inserts E. Producing...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F... F. Infecting Target Cells F.1 Recommended materials F.2 Determining the optimal puromycin concentration...following plasmids available from Addgene are recommended for use in conjunction with the pLKO.1 TRC-cloning...mismatches to all unrelated genes. TIP: Addgene recommends that you select multiple target sequences for...

  16. CRISPR Library Amplification

    Type
    Protocol
    ...(1x10 10 cfu/µg) commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library... pellets for later purification. Immediately purify them! Commercial Maxipreps rely on incremental, ordered...specific pooled library available at Addgene. We recommend following the depositor's specific protocol if... headaches and expense later. At a minimum we recommend the use of a diagnostic digest and high-throughput...specifications described in the equipment manual. Immediately add 1 mL SOC to cuvette. Remove all liquid from...

  17. Pouring LB Agar Plates

    Type
    Protocol
    ...Antibiotic Concentrations Antibiotic Recommended Stock Concentration Recommended Working Concentration Ampicillin...will be screening a large number of colonies, we recommend using larger plates. Many labs use 100 mm x 15...over at high temperature. Pro-Tip Although our recommended temperature should kill most potential contaminants...Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave just a crack...certain your agar is at the right temperature, we recommend using a laser thermometer. Light the flame at...

  18. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...aliquot of your plasmid of interest (see below for recommend amounts) Appropriate restriction enzyme (see manufacturer's...following: DNA Restriction Enzyme(s) Buffer BSA (if recommended by manufacturer) dH 2 O up to total volume Pro-Tips...Restriction Enzyme 3 µL 10x Buffer 3 µL 10x BSA (if recommended) x µL dH 2 O (to bring total volume to 30µL) ...digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least 4 hours. If you ...Restriction enzymes MUST be placed in an ice bucket immediately after removal from the -20 °C freezer because...

  19. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT...forward and reverse primers. Vortex briefly, immediately before use. Use a reservoir and a multichannel...gave reliable and consistent results. Use the recommended plasmid, or test multiple plasmids to find a ...internal reference virus that is 1 x 10 13 GC/mL. We recommend always using a reference within 1-log of the expected... Each curve represents a dilution. References Aurnhammer C, Haase M, Muether N, Hausl M, Rauschhuber C...

  20. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... confirm. Addgene's plasmid information pages recommend 5’ and 3’ sequencing primers. These primers typically...sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the plasmid ... 300 nucleotides from your target. Are there commercially available primers that can be used for sequencing...sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing results as a reference...
Showing: 521 - 540 of 568 results