We narrowed to 602 results for: she

1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ... is generally advised, there is little detail published on how to do it best. Scientists are typically...

3. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   ...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

5. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

6. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

7. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

8. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   ...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

9. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

11. General Transfection

    Type

    Protocol

    ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

12. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

    ...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...

13. An Introduction to Adenovirus

    Type

    Blog Post

    ...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

14. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

15. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

17. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

18. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...