We narrowed to 625 results for: Emb

1. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

2. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

3. Adenoviral Vector Production and Troubleshooting

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   ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

4. Tips for Technical Support Calls

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   Blog Post

   ... money on a potentially faulty product. Please remember that the person at the other end of that email...

5. Common Injection Routes in Mice

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   ...When considering what method you’d like to use, remember that the final decision will likely come down ...

6. Pooled Library Amplifications

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   ... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

7. Antibodies 101: Flow Compensation

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   ...But we hope this guide can help you get started. Remember, you can always change your compensation values...

8. Of Myc and Men

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   ...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984...

9. Transferable Skills: Negotiation

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   ...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ...

10. Truncated gRNAs for Regulating Gene Expression

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    ... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon...

12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

13. 3D Printing Meets CRISPR Cas9

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    ...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

14. Addgene's Cancer Collection Pages Connect the Research Community

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    ... We invite feedback and contributions from all members of the research community as we continue to to ...

15. Experimenting with New Careers while in Grad School

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    ...positive. I went back into lab the next day and remembered why I still love science. Keep building on your...

16. Anatomy of a Plasmid Page at Addgene

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    ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

19. Antibodies 101: Producing Recombinant Antibodies

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    ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

20. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...