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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,076 results for: TOR;

Showing: 811 - 840 of 1076 results

  1. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... plates) and store at -20 °C. Once a standard is thawed do not freeze it again but store at 4 °C and use...SYBR Green Technology You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...the titer of samples obtained from academic viral vector cores. Our titers were similar to those reported...30 min Equipment qPCR instrument Heating plate Pipettors 1–10 µL single channel pipette 20–200 µL single...uL) Volume of nuclease free water (uL) Dilution factor Total dilution Dilution 1 (DNase step) 5 uL AAV...

  3. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency in plants 🎵 ...

  7. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...

  10. CRISPR Library Amplification

    Type
    Protocol
    ...amplification protocols that have been developed by the depositor for a specific pooled library available at Addgene...Addgene. We recommend following the depositor's specific protocol if one is available. If a pooled library...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...amplification protocol is expected to obtain satisfactory results for libraries up to 200,000 individual...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents...time for a total of eight electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm ...

  14. Summer Fun at Addgene!

    Type
    Blog Post
    ...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...

  15. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...

  16. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...Functional data, if the report describes a functional vector such as a channelrhodopsin Images showing the injection...

  22. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... move so fast." On the very same day the monkey story hit the presses, another very different bit of CRISPR...

  28. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...also adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene...and bathroom. These items should remain in the laboratory. When traveling between lab spaces, take your...been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...

  30. Science as Art

    Type
    Blog Post
    ... Geographic and Smithsonian Magazine, where the editor stated “Albert G. Richards, a teacher of dental...
Showing: 811 - 840 of 1076 results