We narrowed to 921 results for: Tro;

1. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...similar to an academic research environment” with a strong focus on publishing, but still made it so she could...

2. Engaging with science and society at pgEd

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   Blog Post

   ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...

4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...boiling point of water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the ...

5. Illuminating Epigenetics with A FRET Based Biosensor

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   ...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

6. How to Prepare for an Industry Interview

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   ...allow it. Before you start your presentation, introduce yourself to your audience. Pay attention to...

7. Plasmids 101: The Wide World of Natural Plasmids

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   ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

8. Making CRISPR Plasmids Using Fragmid

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   ... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...

9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...techniques that you can use antibodies for in our Intro to Antibodies post Resources on Addgene.org Browse...

10. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

11. 10 Basic tips for mammalian cell culture

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    ..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

12. Transferable Skills Guide: Managing a Team

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    ...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

13. Cloning Mammalian Cells with the Agarose Method

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    ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

14. Plasmids 101: Optimizing Plasmid Yields

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    ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

15. Anatomy of a Plasmid Page at Addgene

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    ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    Blog Post

    ...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

17. Viral Vectors 101: Producing Your rAAV

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    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

18. Transferable Skills: Negotiation

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    ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

19. Hot Plasmids: Winter 2025

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    ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

20. Polymerase Chain Reaction Overview and Applications

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    ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

21. Hot Plasmids and Viral Preps - May 2021

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    ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

22. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

23. SciComm: Taking Science to Elementary and Middle Schools

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    ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

24. Course-Based Undergraduate Research Experiences (CUREs)

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    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

25. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...  Susanna Stroik...

26. The Importance of a Fun Workplace: Company Culture at Addgene

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    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

27. A Career in Grants: How To Become a Grant Professional

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    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

28. Plasmids 101: Codon usage bias

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    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...

29. Mesothelioma - Causes, Symptoms, and Treatment

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    ...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...

30. Designing Your Chalk Talk for the Academic Job Interview

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    ...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...