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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,100 results for: Ide

Showing: 1081 - 1100 of 1100 results

  1. Lentivirus Production

    Type
    Protocol
    ...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can...tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone (PES) filter Transfection...the solution. Use hydrochloric acid or sodium hydroxide to adjust the pH to 7.0. Typically, the solution...batch of 1 mg/mL PEI and for each cell line. Considerations Before You Start The health of the packaging...and 1:6. The 1:2 and 1:3 total DNA:PEI μg ratios provided high transfection efficiencies as measured by ...

  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...window) from Sigma-Aldrich. Protocol Video Watch the protocol video below to learn setup and use an iodixanol...Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...can collect fewer fractions once you have a good idea when the virus elutes from the gradients. In a biosafety...tube. (Optional) For first time users, it is a good idea to assay each fraction by silver stain or SYPRO ...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...the virus will have higher levels of expression. Consider expanding populations transduced with a variety...expressors may take over the culture. To overcome this, consider generating monoclonal lines from the early polyclonal... and is described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem...

  4. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Last Update: Feb. 28, 2017 Protocol Video Watch the protocol video below to learn how to isolate single...the plasmid name and the date. It is also a good idea to add the antibiotic resistance and your initials...This dot is composed of millions of genetically identical bacteria that arose from a single bacterium. If...

  5. Cloning

    Type
    Guide
    ..... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used...is likely that for any given project there is an ideal approach. This may be due to speed, cost, availability...method for your research project . The following guide will highlight several of the most popular cloning...DNA that already has restriction sites on either side of it into any plasmid that has the same sites in...sequence, which can make it difficult at times to identify compatible restriction enzymes, that cut your ...polymerases synthesize DNA molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together...

  6. Educational Resources

    Type
    Guide
    ...Fluorescent Proteins 101 Science Career Guide Videos Watch our collection of video content , including protocols...eBooks, science guides, videos, and protocols Educational...Techniques Protocols Find written and video protocols for a wide range of applications in molecular biology... Educational Resources From eBooks to videos and blog posts... to protocols, our educational resources cover a wide range of topics in molecular biology, laboratory...Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth instructional content on subjects...

  7. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Last Update: Oct. 27, 2017 Protocol Video Watch the protocol video below to learn how to spread antibiotic...Several other devices may be used for this purpose, provided that they fit your petri plate. Incubate the plate...

  8. Addgene's eBook Collection

    Type
    Guide
    ...Select Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 |...your gRNA and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent ...Vectors 101 July 2024 | Second Edition About This guide to viral vectors contains an introduction to each... | First Edition About The guide includes information on the many career options in science, advice ...advice on mentoring and management, resources for identifying your transferable skills, and tips on the job...

  9. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically...the ligation process. If the sticky ends on either side of the vector are compatible with each other, the...which degrades upon freeze/thaw cycles, it is a good idea to take a fresh tube, thaw it one time and aliquot...

  10. Colony Formation Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers as the likelihood of multiple...

  11. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Green Technology You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction... 96-well optical plate Pipette tips General Considerations Always run standards and samples in duplicate...small amount of background signal that is most evident during initial PCR cycles. This background signal...

  12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Limiting Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service...monoclonal line could be generated at any time. Considerations Before You Start Some cell lines tend not to... and is described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem...

  14. Weighing Reagents Protocol

    Type
    Protocol
    ...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...of material, use an analytical scale. This will provide more accuracy as these types of scales display ...

  16. Promoters

    Type
    Guide
    ... are located within promoter regions, and they provide a stable binding site for RNA polymerase and transcription...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences. It is not unusual to have...

  17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Pro-Tip DNA resuspension can take time, it is a good idea to let it sit for several hours to overnight at ...at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration ...

  18. Sequencing Primers

    Type
    Guide
    ...Primers? The primer sequences listed on the left are provided for your reference. Addgene does not distribute...primers based on the plasmid backbone and insert. To identify primers that may be useful in your sequencing ...

  19. DNA Quantification

    Type
    Protocol
    ...affected the accuracy of the NanoDrop. It is a good idea to re-zero any spectrophotometer between samples...

  20. Centrifugation

    Type
    Protocol
    ...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...
Showing: 1081 - 1100 of 1100 results