We narrowed to 870 results for: LIC
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TypeBlog Post...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...
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Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...on the advisory board for Art in Giving, a 501c3 public charity raising funds for childhood cancer through... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Developing Transferable Skills During Science Training
TypeBlog Post... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh The Hard Truth About ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his... -
Easing the Protein Purification Process with pCri
TypeBlog Post...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634.... -
Science as Art
TypeBlog Post... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...Brazil. Through an expansion of our export license application service, we’re pleased to announce that we... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... -
Build Your CRISPR Vocabulary
TypeBlog Post...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... a Cas9 nickase and two gRNAs. For nickase applications, a common question is: how should the gRNAs ...information and a link to the archived webinar, click here. We would like to thank IDT scientists Mollie... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...contain splice acceptors. They require insertion into an intron of a gene, where they can be spliced into... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene. CRISPR-Act3.0, ... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...takes minutes. As a result, it is not possible to elicit an immediate response or track the exact time of...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...