We narrowed to 589 results for: Ret;

1. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...

2. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...

3. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   ...drawback for therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   ...color for its entire lifetime and daughter cells retain the same color, allowing for the fate mapping of...

5. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   ...Spinach, a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...by Wrenbeck et al but it’s likely that longer stretches of sequence could be used. However, a practical...

7. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   ...PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in...

8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   ...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching...

9. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   ...of protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013):...

10. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    ...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...

11. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...). E. coli as a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net...

12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma...

13. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    ...academic research. Panelist Moon Duchin provided a concrete example of how underrepresented individuals are...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    ...and -2. The general structure of Brainbow-1.0 is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2...

15. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...proteins responsive to light in combination with retinal) variations designed to give researchers flexibility...

16. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    ... patterns with several overlapping targets to interpret. Check out (Jambor et al., 2021) for more guidelines...

17. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    ...9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer, Barret D., et al. “Refinement of Tools for Targeted Gene...

18. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1...

19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ...notebooks also play a role here by making it easier to retain searchable, thorough notes on how lab procedures...

20. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    ...friend didn’t heed this warning and profoundly regretted it when the same thing happened to her a year ...