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We narrowed to 818 results for: eng

Showing: 541 - 560 of 818 results
  1. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...
  2. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...
  3. Plasmids 101: FLEx Vectors

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    Blog Post
    ...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...
  4. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...
  5. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...
  6. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...
  7. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...
  8. Transferable Skills: Negotiation

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    Blog Post
    ...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...
  9. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ...entire time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual...they are following along. This will not only re-engage you with those who sought out your poster, but ...
  10. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...
  11. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...models in species that were previously difficult to engineer, like rats. If you have tools for creating knockout...
  12. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...
  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...
Showing: 541 - 560 of 818 results