...light instead of arabinose. Nat Chem Biol. https://doi.org/10.1038/s41589-021-00787-6 Additional resources...the Plasmids topic overview page Resources on Addgene.org Check out the Molecular Biology Reference Download...

...Immunoassay Handbook (pp. 245–265). Elsevier. https://doi.org/10.1016/b978-0-08-097037-0.00017-8     Morelock...Issue 17, pp. 13048–13055). Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources on...

... frankenbody can be used for imaging in whole organisms. The researchers used the HA frankenbody to successfully...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

...and zebrafish – essentially all of the standard organisms for which extensive genetic tools are available... Kendall Morgan...

...Girls) Communication to diverse audiences Organization Mentorship Leadership Science writing...Communication Business models Conference or club organizer Management Leadership Communication ...

...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...

...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

...enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in...Plasmids by Colony PCR Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs...

...Proteins on the other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...

...something. Offer to help out wherever you can and don’t forget to express your gratitude. Be the mentee that YOU... YOU would like to train! And, of course, don’t forget to have fun! Visiting is an amazing career opportunity...

...available, just ask an Addgene scientist at help@addgene.org! References and Resources  References Joung ...Lentiviral Transduction Additional Resources on Addgene.org Plasmid Pooled Libraries Pooled library amplification...

...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...

... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...visualize the DNA under ultraviolet (UV) light. Don’t forget to add loading buffer to your digest reactions ...

...The experiments must fit with their interests.” George Church selects new hires initially based on those... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...

...check out our updated CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational...import of newly synthesized (green) protein and organelles with older (yellow to orange) protein. Image ...favorite plasmid technologies? Email us at blog@addgene.org  and let us know what plasmids you would like...

...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...

...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline...