We narrowed to 883 results for: prot

...only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features, such as...between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align...

...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated...

...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

...sensitivity are key considerations in choosing a Cas protein for your experiment. References Hu, Johnny H....101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources on Addgene.org...

...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

...strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will require...A: At CLOVER, we have traditionally used qPCR (protocol link). We have found this method to be reliable...

...to be due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson...virus-specific elements such as the LTRs or structural proteins are not interchangeable between the two systems...

... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...

...most recombineering strategies, only 3-4 phage proteins are needed and these can be provided via a separate...transformed into bacteria expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous...

...factor expected to impact repair is that the Cas9 protein doesn’t immediately release from the broken end... CRISPR 101: Homology Directed Repair CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines ...

...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

... plasma membrane localization of transmembrane proteins, like rhodopsins, are highly constrained and poorly...design new channelrhodopsins with high-throughput proteins engineering methods, like directed evolution, ...

... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...

...require an ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional...

...Check out our AAV Production Protocol Check out our AAV Purification Protocol ...

...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...

...exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed...response set off by a DREADD depends on what G-protein and, consequently, what pathway the receptor acts...

...molecularly characterized by misfolding of the TTR protein, which contributes to the disease phenotype. Intellia...showed a high success rate in clearing misfolded protein levels as measured in the blood. More information...