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TypeBlog Post...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...
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A Guide to Starting Your Own Journal Club
TypeBlog Post...stay up to date and engaged with new advances in published science. They also serve as a way to learn about... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide... -
CRISPR Antimicrobials
TypeBlog Post...antibiotic resistant bacteria have previously flourished. Figure 2: Two-phage strategies to resensitize... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post..., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...our educational resources. From 2013–2023, we published 110 videos, which received over two million views... -
How to Write a Scientific Review Article
TypeBlog Post...include every piece of information that was ever published. Obviously this isn’t possible. So, define your... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...express Anabaena GVs in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...have been used successfully in experiments and published in peer reviewed journals. If you need to generate... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...might help to get you started. Once you have established a well working protocol in your lab, it can become... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene.... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...