We narrowed to 625 results for: Emb

1. AAVs in Retinal Gene Therapy

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   Blog Post

   ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

2. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... Science Night, a chance for parents and other members of the community to share their expertise with ...

3. Summer SciComm Series: Modes of Communication

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   Blog Post

   ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

5. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

6. Cpf1: A New Tool for CRISPR Genome Editing

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   ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

7. Hot Plasmids Spring 2024

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   Blog Post

   ...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...

8. Plasmids 101: Plasmid Incompatibility

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   ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

9. Transferable Skills Guide: External Collaborations

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   ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

10. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category...

11. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc...

12. Career Insights: Technical Support Specialist

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    Blog Post

    ...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced...

13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

14. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

15. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

16. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus...

18. Viral Production at Addgene

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    Blog Post

    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

19. New CRISPR Tools: Cas7-11 and PASTE

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    ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

20. Antibodies 101: Immunoprecipitation

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    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...