We narrowed to 958 results for: ARC
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TypeBlog Post...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method. Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...
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Transferable Skills Guide: Cross-team Communication
TypeBlog Post... long tenure at Addgene. I would work with our research team and software engineers to update our Lab ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post... School of Medicine and a researcher at the Yerkes National Primate Research Center. Using a combination...combination of in vivo and anatomical techniques, her research aims to gain a deeper understanding of the physiology... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post... podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what... Yeast Transcription Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids...that this level of control will enable future researchers to better simulate physiological gene expression... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,... -
Plasmids 101: Cre-lox
TypeBlog Post...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post..., Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, .../10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...only for translational research, such as gene therapy, but also basic research. To improve cell-type specificity...valuable tools for basic as well as translational research for gene therapy. The Roska lab hopes to design... -
Tips for Technical Support Calls
TypeBlog Post... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...working at the Association for Research in Vision and Ophthalmology Research (ARVO). Hopefully sharing my... in closer connection with other leaders in my research field should I decide to remain here. In a time... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business... -
Common Injection Routes in Mice
TypeBlog Post...drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior...commonly administered through the tail vein — many researchers will simply refer to these as “tail vein injections...feeding tube can be flexible or metal, depending on researcher preferences. It sounds complicated but has a ... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus...sybody libraries in the form of mRNA for academic research. The template plasmids for generating your own...days. While the lab used this method for COVID-19 research, this protocol holds promise for developing treatments... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post... and what is their background relative to your research? Tailor your language to your audience so that...presenting at the American Association for Cancer Research Annual Meeting, you might consider including more...group who presented on a topic related to your research? Find a way to get in contact with the presenter... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your...all will be familiar with the specifics of your research. Try to avoid jargon in this initial introduction...data they may ask the audience to not share their research on social media. Use the hashtag if you are live-tweeting... -
Academic vs. Industry Postdocs
TypeBlog Post... essential part of being able to continue your research. As an academic postdoc, you’re often required... at once at the end of February or beginning of March, and the jobs are mixed into the general careers...developmental biology and is applying this towards researching neurodegenerative diseases. Additional resources... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...depositing them in Addgene’s repository. Other researchers, eager to try this new technology, were quick...the CRISPR community? Addgene exists to help researchers share useful tools, and as such, we’re always...collection, we’ve shared almost 300,000 plasmids with researchers all over the world. That’s an average of 27,272...