We narrowed to 591 results for: ARL

1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   Blog Post

   ... or excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-...

2. Deep Dive: Statistical Tests (Comparisons)

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   Blog Post

   ...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

3. Important Considerations When Using AAVs

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   ...receptors (i.e. chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic...

4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   ...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...

5. Sequencing Options for CRISPR Genotyping

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   Blog Post

   ...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...

6. Typing CRISPR Systems

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   ...about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely...

7. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

8. Antibodies 101: Introduction to Antibodies

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   Blog Post

   ...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...

9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   ...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...

10. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post

    ...tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ...

11. Custom CRISPR Screens & the Green Listed Software

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    ... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified...

12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching...

13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    ...stable signal over extended periods, which is particularly beneficial when studying dynamic processes like...

14. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

15. CRISPR 101: Multiplex Expression of gRNAs

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    ...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

16. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation in vitro where...

17. Antibody Validation for Flow Cytometry

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    Blog Post

    ...different providers for the same clone. This can be particularly useful when designing larger multicolour panels...

18. Delivery Methods for Generating iPSCs

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    ...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent...

19. Technical Design of a Western Blot

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    Blog Post

    ...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...

20. Adenovirus Guide

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    Guide

    ... divided into early (E1A, E1B, E2, E3, E4) and late (L1, L2, L3, L4, L5) regions. Early transcripts are...5 (Ad5) and have been produced by replacing the early and late adenoviral transcript regions between the...rAdV vectors, as well as the deletion of the other early gene regions, E2 and E4. These modifications aimed... when administered in vivo, however deletion of early genes also resulted in a reduction of viral vector...Adenoviral plasmid E2A, E2B, E3, E4 in trans Adenoviral early transcript regions involved in viral DNA replication...