Addgene

We narrowed to 576 results for: ARL

Showing: 561 - 576 of 576 results

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Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...
Sequencing Options for CRISPR Genotyping

Type

Blog Post

...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

...leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Type

Blog Post

...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...
Typing CRISPR Systems

Type

Blog Post

...about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Type

Blog Post

...stable signal over extended periods, which is particularly beneficial when studying dynamic processes like...
Which Fluorescence Microscopy Techniques is Best for Me?

Type

Blog Post

...tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ...
Antibodies 101: Introduction to Antibodies

Type

Blog Post

... Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

...38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation in vitro where...
Antibody Validation for Flow Cytometry

Type

Blog Post

...different providers for the same clone. This can be particularly useful when designing larger multicolour panels...
Delivery Methods for Generating iPSCs

Type

Blog Post

...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent...
Technical Design of a Western Blot

Type

Blog Post

...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...

Showing: 561 - 576 of 576 results

Results per page:

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Sequencing Options for CRISPR Genotyping

Custom CRISPR Screens & the Green Listed Software

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Typing CRISPR Systems

Antibodies 101: Designing Your First Flow Panel

Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Which Fluorescence Microscopy Techniques is Best for Me?

Antibodies 101: Introduction to Antibodies

Special Delivery: Fluorophore Targeting for FRET Studies

CRISPR 101: Multiplex Expression of gRNAs

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Antibody Validation for Flow Cytometry

Delivery Methods for Generating iPSCs

Technical Design of a Western Blot

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