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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 561 - 580 of 856 results

  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that...emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation at another...2023). The field of fluorescent ligands and dyes has witnessed remarkable advancements, driven by the ...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... crucial environmental research programs, Canada has been here before.  These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many...

  13. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...

  14. Transferable Skills: Negotiation

    Type
    Blog Post
    ...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

  15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

  16. Of Myc and Men

    Type
    Blog Post
    ...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

  17. PhD Applications After COVID

    Type
    Blog Post
    ... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...
Showing: 561 - 580 of 856 results