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  2. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get...

  4. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  6. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  7. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

  20. Antibodies 101: ChIP

    Type
    Blog Post
    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...
Showing: 561 - 580 of 858 results