Plasmids 101: Shuttle Vectors
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Blog Post
...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living...
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CRISPR 101: Any Base Transversion Editors
TypeBlog Post...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...our educational resources. From 2013–2023, we published 110 videos, which received over two million views... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...express Anabaena GVs in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors... -
How to Write a Scientific Review Article
TypeBlog Post...include every piece of information that was ever published. Obviously this isn’t possible. So, define your... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian... -
Protocol - How to Run an Agarose Gel
TypeProtocol... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid... -
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)
TypeProtocol...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual... -
Five Popular Model Organisms, Part 2
TypeBlog Post...with their regenerative abilities is expected to shed light on human aging pathological developments (... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker... -
Hot Plasmids: Summer 2024
TypeBlog Post..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...