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Showing: 561 - 580 of 594 results

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Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

...reliable in the future. We hope that this post has shed some light on immunofluorescence. The technique ...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...
Five Popular Model Organisms, Part 2

Type

Blog Post

...with their regenerative abilities is expected to shed light on human aging pathological developments (...
Lentivirus Production

Type

Protocol

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and variations...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...
Plasmids 101: Restriction Cloning

Type

Blog Post

...ratio” in the ligation reaction. 5. Purify the finished plasmid Once it looks like your ligation has...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...
AAV Purification by Iodixanol Gradient Ultracentrifugation

Type

Protocol

...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...
Protocol - How to Run an Agarose Gel

Type

Protocol

... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges...
Handling Plasmids from Addgene - Purifying Plasmid DNA

Type

Protocol

...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid...
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Type

Protocol

...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual...
Quick Guide to All Things Lentivirus

Type

Blog Post

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

Showing: 561 - 580 of 594 results

Results per page:

Fluorescent Proteins 101: When GFP lets you down

Transferable Skills Guide: Conflict Resolution

Antibodies 101: Introduction to Immunofluorescence

Harnessing Bacterial Toxins for Allelic Exchange

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Five Popular Model Organisms, Part 2

Lentivirus Production

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Antibodies 101: Multiplex Immunofluorescence

Generating Mouse Models Using CRISPR/Cas9

Plasmids 101: Restriction Cloning

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

CRISPR 101: Epigenetics and Editing the Epigenome

AAV Purification by Iodixanol Gradient Ultracentrifugation

Protocol - How to Run an Agarose Gel

Handling Plasmids from Addgene - Purifying Plasmid DNA

Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Quick Guide to All Things Lentivirus

When Fidelity Matters: A frank discussion about ligase fidelity

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