We narrowed to 605 results for: SHE

...might help to get you started. Once you have established a well working protocol in your lab, it can become...

...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

..., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris...

...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ...

...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...

... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges...

...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid...

...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual...

...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...

...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

...case, however, and recent work has already been published on improving base editing precision. Similar treatments...

... Alyssa Shepard...

..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...

...with their regenerative abilities is expected to shed light on human aging pathological developments (...

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...