We narrowed to 954 results for: org

1. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   Blog Post

   ...were facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to ...same time. I began approaching science funding organizations to request support for our operations until ...

2. Antibodies 101: Chimeric Antibodies

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   Blog Post

   ...Immunoassay Handbook (pp. 245–265). Elsevier. https://doi.org/10.1016/b978-0-08-097037-0.00017-8     Morelock...Issue 17, pp. 13048–13055). Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources on...

3. Plasmids 101: TOPO Cloning

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   ... reaction is fairly quick, make sure you stay organized and prepare everything you need for the next step...Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up...

4. Hot Plasmids: FRET-Based Biosensors

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   ...and zebrafish – essentially all of the standard organisms for which extensive genetic tools are available... Kendall Morgan...

5. Extracurricular Activities for a Strong Science Career Path

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   ...Girls) Communication to diverse audiences Organization Mentorship Leadership Science writing...Communication Business models Conference or club organizer Management Leadership Communication ...

6. Creating accessible biology activities in schools with BioBits

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   Blog Post

   ...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

7. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   ...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...

8. New and Upcoming Viral Vectors - June 2019

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   ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...

9. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   Blog Post

   ...Proteins on the other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...

11. Pushing the Limits of DNA Assembly

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    Blog Post

    ...enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in...Plasmids by Colony PCR Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs...

12. Trading Spaces - Visiting Scientist Edition

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    Blog Post

    ...something. Offer to help out wherever you can and don’t forget to express your gratitude. Be the mentee that YOU... YOU would like to train! And, of course, don’t forget to have fun! Visiting is an amazing career opportunity...

13. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

    ...available, just ask an Addgene scientist at help@addgene.org! References and Resources  References Joung ...Lentiviral Transduction Additional Resources on Addgene.org Plasmid Pooled Libraries Pooled library amplification...

14. How to Lead a Great Meeting

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    Blog Post

    ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

15. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    ...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...visualize the DNA under ultraviolet (UV) light. Don’t forget to add loading buffer to your digest reactions ...

16. Finding and Joining Your Dream Lab

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    Blog Post

    ...The experiments must fit with their interests.” George Church selects new hires initially based on those... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...

17. 22 Hot Plasmid Technologies from 2014

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    Blog Post

    ...check out our updated CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational...import of newly synthesized (green) protein and organelles with older (yellow to orange) protein. Image ...favorite plasmid technologies? Email us at blog@addgene.org  and let us know what plasmids you would like...

18. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

19. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post

    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

20. How Addgene Creates Content

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    Blog Post

    ...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles answering...reach out about writing a guest post (email news@addgene.org.) We are here to support and advance the scientific...