We narrowed to 883 results for: prot

...enzymatic machinery (i.e. RNA replication with no proteins). The chemistry is complex and this work requires...system. His lab is also exploring the use of cas9 proteins from different species for genome engineering....

...the looping of chromatin, the complex of DNA and proteins that forms chromosomes. These long range DNA interactions... of the key steps of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested...

...tissues, eliminating the need to deliver the Cas protein altogether. Platforming AAV Interest in interrogating...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...

... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...multiple PAMs should have a fitness advantage over proteins with single PAM recognition. Created with...

...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

... can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...

...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002...

...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, which deaminate...

...previous books focused primarily on fluorescent proteins and their applications, tackles much broader issues...scientists, the identification of Green Fluorescent Protein (GFP) and its development into an essential tool...

... BCA assay uses a serial dilution of a control protein, incubated alongside an unknown sample with the... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

...interacting with proteins such as transcription factors and chromatin modifiers. These proteins recognize and...

...or other plasmid part? Do you want to fuse your protein to any tags or fluorescent markers? If you’re creating... streamline your plasmid extractions. Use this protocol shared by Dr. Rob Hurt for high-throughput minipreps...

...for multicopy chromosomal integration (ex: for protein expression) (Zhang et al., 2020). Multicopy chromosomal...pQCascade expresses the transposase TniQ, the Cas proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of...

...inhibiting translation of replication machinery proteins, or directly inhibit replication by binding to... the plasmid, a centromere-like region binding protein (the CBP), and a partition NTPase (Schumacher, ...

... has 10 gRNAs per gene and targets all ~20,500 protein-coding genes. Unique, non-overlapping sites in ...: 1) targeting guides (blue), which target all protein-coding genes present in the genome; 2) non-targeting...

...Resources at Addgene.org Check out our Protocol Pages for Video Protocols ...

... editing in stem cells using CORRECT." Nature protocols 12.2 (2017): 329-354. PubMed PMID: 28102837. Additional...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

...present that vary in cell type, size, and marker proteins. But even if you are analyzing a homogenous cell... only enter dead cells, while living cells can protect themselves from it. So here, I actually want to...

...   Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how... such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase (TH), can limit...

...amount of information regarding which regulatory proteins, signaling molecules, mutations, and environmental...combination of red, blue, and green fluorescent proteins in each state. The three methods of state interrogation...