We narrowed to 653 results for: EPO
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TypeBlog Post...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Listen to this episode...regulate botulinum neurotoxin were also created. The depositors demonstrated the utility of light-mediated suppression...Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian...contributed by Lukas Morgan Listen to the acoustic reporter gene podcast segment In recent years, scientists...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Human whole genome sgRNA...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are... -
Depositor Collections
TypeCollection...Plasmid Collections Depositor Plasmid Collections Depositor Collections Addgene's repository contains 159,430...collections from depositors. To help you find what you're looking for, we've gathered our depositor collections...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression... -
CRISPR Library Amplification
TypeProtocol...amplification protocols that have been developed by the depositor for a specific pooled library available at Addgene...Addgene. We recommend following the depositor's specific protocol if one is available. If a pooled library... Qubit. Each different DNA measurement type can report slightly different concentrations of DNA. In our...our hands fluorescent dye methods tend to report lower values than Nanodrop, although most protocols have... -
Video Library
TypeProtocol...Help Center How to Deposit Your guide to depositing plasmids with Addgene Depositing Page Career Videos... -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocol...vector cores. Our titers were similar to those reported by these institutions. This protocol is for a ...plasmids to find a suitable one. Some labs have reported better results when the plasmid is linearized.... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may also be useful for...Material Transfer Agreements between the party that deposited the material at Addgene and the party receiving... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol...been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...