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We narrowed to 160 results for: ALC

Showing: 41 - 60 of 160 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ...it to create a standard curve on your blot to calculate the amount in your sample. (Do ensure your blot.../Reprobe PVDF membrane Increased time Calculate absolute quantification Running a standard curve...wells; purified protein not always available Calculate relative quantification Normalizing to ubiquitously...

  2. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ... CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...target. Read the paper in Development Find the Valcyrie plasmids If you have a new CRISPR tool you’ve...

  3. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product packaging) for free! It has... If you use serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue...

  4. Optogenetics Guide

    Type
    Guide
    ...-encoded reporters of molecular signals; e.g., calcium indicators In this overview, we will focus on the... truncated forms of cytosolic STIM1 to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing...light-controlled molecules. ~450–495 BACCS light-activated calcium channel switch iLID Improved light-inducible dimers...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

  17. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

  20. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...
Showing: 41 - 60 of 160 results