We narrowed to 103 results for: EXO

1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   ...immunoblotting to detect. Another approach is to target two exonic sites within a single gene using paired DSBs. This...

2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   ...implant Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled...

3. FastCloning: A Newer, Simpler Cloning Technique

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   ...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...

4. What the HEK?

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   ...Membrane receptors and ion channels are often exogenously expressed and studied in HEKs due to the cell...

5. Advice for Scientists Starting a Lab

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   ...Professor who was always “off flying his plane to exotic places while occasionally emailing us his worldly...

6. Viral Vectors 101: Biosensors

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   ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

7. GCE4All: Making Genetic Code Expansion Accessible

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   ...because it uses ∆serB strains, does not require exogenously added phosphoserine. A community resource Like...

8. Viral Vectors 101: Viral Applications

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   ...specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for example. Some customization...

9. CRISPR 101: Non-Homologous End Joining

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   ... it may be possible to direct insertion of an exogenous DNA fragment at a Cas9 targeted break (or pair...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...co-infected into the cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond...

11. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...pathogens. Clift et al. found that addition of exogenous TRIM21 protein along with an antibody targeting...

12. What's New in CRISPR - November 2021

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    ...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

13. Human Germline Editing Using CRISPR

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    ... editing sites in the embryos, as well as whole exome sequencing of embryo derived-ES cells.They also ...

14. CRISPRainbow and Genome Visualization

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    ...labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific and sensitive...

15. All in a Twist: dsRNA

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    ...with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA...

16. Plasmids 101: Golden Gate Cloning

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    ...a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20...

17. Adenoviral Vector Production and Troubleshooting

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    ... made of 3 types of proteins: fiber, penton and hexon proteins. The viral genome DNA is further protected...

18. Making CRISPR Plasmids Using Fragmid

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    ...destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    .... Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...-white screening require X-gal, an expensive exogenously-added substrate, chromoproteins don’t require...