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We narrowed to 65 results for: c myc del 2

Showing: 61 - 65 of 65 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... fluorescent proteins expressed in the specimen.  2. Fluorescence Activated Cell Sorter (FACs) A machine...photostability of your fluorescent protein when using 2-photon microscopy as this hasn't been well reported...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role...PMID: 18703742. PubMed Central PMCID: PMC2606057. 2. Chtanova, Tatyana, et al. "Dynamics of neutrophil...help immunologists create even better tools and models to further our understanding of the immune system...during the development and disintegration of mycobacterial granulomas." Immunity 28.2 (2008): 271-284. ...presentation and T cell effector function in mycobacterial granulomas." Immunity 34.5 (2011): 807-819. ...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... easy for a researcher to quickly generate N- and C-terminal epitope tag fusions to a protein of interest...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...quickly study your gene of interest in a variety of model systems, thereby opening your research to a variety...initiation heavily dependent upon the core of a Shine-Delgarno sequence found at the 3’ end of the leader sequence...predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called bicistronic design...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance....

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...changed every 2–3 days to maintain the dose of antibiotic, which may not be stable at 37 °C. To achieve ...Workflow Timeline Day 0: Seed and transduce Cells Day 2–3 (am): Remove media, replace with fresh media containing...Biological Safety Cabinet 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel... 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can promote...it can be inactivated in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health...confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method results...

  4. Lentiviral Vector Guide

    Type
    Guide
    ..., Hahn, W. C., Sharp, P. A., Weinberg, R. A., & Novina, C. D. (2003). Lentivirus-delivered stable gene...., & Baum, C. (2013). Biosafety features of lentiviral vectors. Human Gene Therapy , 24 (2), 132–142. ...intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene and wild-type...envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created with...plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains env Third-generation...The inside out of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: ...PMID: 33768124 Marshall, H. M., Ronen, K., Berry, C., Llano, M., Sutherland, H., Saenz, D., Bickmore, ...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or...for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com...Wong, G. K., Cameron, E. R., Kilbey, A., & Neil, J. C. (2016). Gamma-Retrovirus integration marks cell Type-Specific...10.1371/journal.pone.0154070 PMID: 27097319 LaFave, M. C., Varshney, G. K., Gildea, D. E., Wolfsberg, T. G....gkt1399 PMID: 24464997 Maetzig, T., Galla, M., Baum, C., & Schambach, A. (2011). Gammaretroviral vectors:...Rubat, L., Nikoniuk, A., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J...for stable delivery of various genes, mutations, or treatments into cells, and has been widely used in the...
Showing: 61 - 65 of 65 results