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We narrowed to 311 results for: cas9 genes

Showing: 61 - 90 of 311 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... and the wild-type sequence persists, Cas9 will cleave the gene of interest, inducing a lethal DSB.   ...Jiang et al., 2015). pCas contains Cas9 and the phage recombination gene lambda Red. pTargetF contains the...pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces-2 contains Cas9 and a gRNA cassette...functions more similarly to CRISPR/Cas9 systems adapted for eukaryotes in that Cas9-mediated cleavage induces ...efforts to regulate gene expression were limited to inducible promoters or direct gene knockout. In contrast.... These could be genes, specific polymorphisms, including antibiotic resistance genes. This approach successfully...The system available from Addgene consists of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance...

  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms ... as ‘indels’. While this is convenient for generating gene knockouts, NHEJ repair does not allow introduction... and found a general relationship between the distance of a mutation from the CRISPR/Cas9 cut site and... Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your ...organisms and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific ...induced pluripotent stem cells (iPSCs). The CRISPR/Cas9 com­plex is very efficient at introducing double...Cas-blocking mutations After genome editing with CRISPR/Cas9 in human cells was described in 2013 (Cong et al...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...shifts. This backbone model of Cas9 is based on Fuguo Jiang’s structure of Cas9 – primed for cleavage. This...of all proteins in general, and the amazing conformational flexibility that the Cas9 protein must have ...CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR...contacted us in 2014 to ask if we could make a model of Cas9 based on the structure published by Martin Jinek...other aspects of the structure. While this first Cas9 model is certainly useful, it is also wrong in at... at least one serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible...model of a protein. We now have another model of Cas9 – a flexible alpha-carbon backbone model made of...

  4. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed to create a system...: Can you engineer CRISPR-Cas9 activators that work well enough on any gene that they could be used by... endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion;...system also enabled them to turn on genes anywhere in the genome for gene control over a 1,000-fold range....First of all, the Cas9s can be turned on and off, to reversibly activate or repress genes of interest. They...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...

  5. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans, whose optimal...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 from ...30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? ...blog posts Read about GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide...launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn... Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active ...Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of many “dark...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...prime editor consists of a Cas9 nickase fused to a reverse transcriptase. The Cas9 nickase is guided to the... that most Cas9-induced DSBs are repaired by non-homologous end joining. As a result, Cas9 treatment causes... ~ ~ ✓ ✓ ✓ R221K N394K Cas9 mutations Improve Cas9 nuclease activity ~ ~ ~ ~ ...has been widely used to generate precise changes. However, the efficiency of Cas9 cleavage is relatively...homology to the genomic DNA locus. After nicking by Cas9, the homologous pegRNA sequence hybridizes to the...-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase (PE1). But innovations by the Liu ...ratios of desired edits to indel byproducts than Cas9-initiated HDR. Prime editing tools Prime editing...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...C-terminus of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the...tested plasmids that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are... Figure 1: Gene editing is the addition of a useful coding sequence (i.e. GFP) into a gene while leaving...leaving other features of the gene intact. Here, we show an example gene targeted for tagging with GFP at...allele, or gene copy. The coding sequence for GFP is transcribed and translated along with the gene, producing... CRISPR/Cas9 to make a precise cut in the genome near either the beginning or end of the gene of interest...tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging systems...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...enrich for Cas9 variants with activity on non-NGG PAMs, generating xCas9 3.0-3.13. To test xCas9 3.0-3.13...available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered Cas9 PAM Variants...select for Cas9 mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick...of the gene of interest. They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9...protospacer. If ω-dCas9 could recognize a library PAM, it would induce transcription of gene III, permitting...In order to bind DNA, Cas9 and other CRISPR enzymes require a short PAM sequence adjacent to the targeted...problem, researchers have engineered alternative Cas9s binding distinct PAM sequences. Now, Hu et al., ...

  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for...your target CRISPR/Cas9 is often used to study the biology surrounding a particular gene (although it has... this gene. Should you cut it at the beginning of the coding sequence to delete the entire gene? Perhaps... encoding Cas9 into the oocyte. This original method was developed as the use of CRISPR/Cas9 in mammalian... in vitro, and using it in mouse embryos to generate gene modified mouse lines. Hopefully this post will...they have been generated (more on that later). Find Validated gRNAs to Target Mouse Genes To begin the ...chances of gene modification in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9...

  10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to...Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches to enable rapid...physiological gene expression regimes that cannot be easily achieved with classical gene overexpression... to study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety...quantitative analysis of epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found here...contain changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not ...Communications. 2017. PubMed PMID: 28924139. FIRE-Cas9 for inducible interrogation of the epigenome Article...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... tags for live imaging in C. elegans CRISPR/Cas9 for gene disruption in M. abscessus  Bioluminescent reporter...has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an...the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...Overview of the dual-plasmid CRISPR/Cas9 workflow in M. abscessus. A) Sth1Cas9 mediates double strand break ...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah Hung’s lab has...

  12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...working in China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later...editing, there was a generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This...valid critiques being offered of He’s work: CRISPR/Cas9 genome editing is not far enough along, scientifically...research published on December 13 shows us, CRISPR/Cas9 techniques are continually being refined; it is ...an embryo—even if it’s not necessarily via CRISPR/Cas9. This leaves us with the second critique. Absent...emerging technology. We could do this for CRISPR/Cas9 – or any other germline editing technique—too. As...not “what is the translational pathway for CRISPR/Cas9 research” but whether or not we will stop shuffling...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...works in general. Base editors use either catalytically dead Cas9 (dCas9) or a Cas9 nickase (nCas9) to avoid...need from Cas9 are sequence specificity and the R-loop structure. In order to bind DNA, Cas9 requires ...non-target strand of DNA for Cas9 to bind.   R-loop The structure formed by Cas9 binding to DNA and separating... Editing CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...PAM) on the opposite strand. Once it finds a PAM, Cas9 unwinds the adjacent DNA to create a region of separate...single-stranded DNA (ssDNA) region, using enzymes fused to Cas9 to alter the exposed ssDNA bases. Thus, base editors...

  14. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ... they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling...sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas...awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and Dr. ...offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus programs work... crispr/cas9.... Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...

  15. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...re-targeting after HDR. If your Cas9 cuts in the coding region of a gene, make sure that the PAM edit you...-in mutations and gene editing Targeted genome editing events fall into two general classes: knock-in ...as swapping out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting..., S., Brion, A., et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair...Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage ...inhibitors to your culture media prior to and after Cas9 introduction increases HDR, just make sure to remove...protein to the nuclease. Early HR factors fused to Cas9 have been shown to increase HDR frequency by committing...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ..., a base editor containing high fidelity Cas9 variant HF-Cas9. They subsequently tested the original BE3...-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3  VRER-Cas9 (NGCG)   ...understanding of B. subtilis gene functions and interactions. The single-gene deletion library is derived...studying the phenotype of an essential gene, the CRISPRi Essential gene Knockdown library was constructed ...The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase for targeted...Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3... pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3  VQR-Cas9 (NGA)  Lower off-target activity than...

  17. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...PX458), pSpCas9n(BB)-2A-GFP (PX461) expresses both a Cas9 protein and a gRNA. But instead of Cas9, this ...Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use...already expressing Cas9. Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro ...HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an ...Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang lab expresses Cas9 from Streptococcus...This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9 and your... over 40,000 CRISPR-Cas9 components over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9...

  18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: ...editing single genes is very useful, sometimes, you need to modify multiple genes to generate a cellular ... constructs using a catalytically “dead” S. pyogenes Cas9 (dC9-ABE8.x-m/d). The dC9-ABE8.x-m/d constructs...adenine base editors The most commonly used S. pyogenes Cas9 requires the target site in the genomic DNA ...Nicole Gaudelli, the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a ...frequency more than 90% compared to ABE7.10. Choosing Cas9 proteins to increase the targeting scope of adenine...: NG) to create NG-ABE8.xm/d and the S. aureus Cas9 (PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these ...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...., et al. "Evaluation of TCR gene editing achieved by TALENs, CRISPR/Cas9, and megaTAL nucleases." Molecular... the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse...triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 (2015): 407-415. PubMed PMID: 25566793...strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing." BMC genomics 15.1...genome-wide specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID...Mouse Models with CRISPR/Cas9 Additional Resources on Addgene.org View Our CRISPR Guide Pages Find CRISPR...off-target mutation and not an edit in the target gene. The validation process, also known as CRISPR genotyping...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fully functional Cas9 upon dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal... cassette to the gene of interest. The gene to be expressed is amplified using a gene-specific forward...at blog@addgene.org and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein ...Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics is a powerful tool that... 10.1073/pnas. CRISPR-Cas9 optogenetic tool for activating endogenous genes Moritoshi Sato's Lab has... the bi-lobed architecture of Cas9 to engineer a series of "split" cas9 molecules that cannot function...precise temporal control of gene knockout or activation, the c-terminal cas9 fragment was fused with FK...

  21. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ..., a base editor containing high fidelity Cas9 variant HF-Cas9 (Rees et al., 2017). HF-BE3 showed 37-fold...other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets with a 14% indel...editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine...individual Cas9. They also mutagenized the cytidine deaminase portion of the base editor to create SpCas9 base...is important because ABEs have generally been slow, meaning that the Cas9 domain often lets go of the DNA...et al., 2020 SpRY Cas9, available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM... 1.1%. These numbers are a vast improvement over Cas9-mediated HDR for the loci tested, where average ...

  22. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of...bound to the DNA. Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better...considered weak, especially compared to the power of Cas9. However, by continually passing the ssDNA past ...better option. To get large deletions using Cas9, two or more gRNAs must be used along the same chromosome... with expressing all Cascade components can make Cas9 easier to work with than Cas3. Future targets Cas3...therapeutics. Imagine deleting a problematic copy of a gene that causes a debilitating disease! This is, of ... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...

  23. Editor's Choice, July 2016

    Type
    Blog Post
    ...from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update...made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might... when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered ...the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals Recombinase-based State Machines... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...

  24. Video Library

    Type
    Protocol
    ...Deletions with CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions... Educational Resources Addgene Videos Addgene Videos Browse Addgene's collection of video content...using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions for...samples from Addgene Technology Transfer How to Order A step-by-step walkthrough of Addgene's ordering process...this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica...she loves about working at Addgene! Maria Soriano Maria Soriano, from Addgene's UK office, discusses her...

  25. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ... found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically more favored...are leading the edge of the precision gene editing. CRISPR-Cas9, the key component to enable efficient...also exhibited reduced Cas9-dependent off-target editing and do not cause higher Cas9-independent off-target...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially... number of genes with a single viral vector.   Fig. 1: Schematic of multiplex gene editing with...enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and ...demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular dystrophies...

  26. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have...and find several mutations in the same gene or in multiple genes, all of which seem to contribute to a ... kit to CRISPR/Cas9? When would you choose one or the other? Musunuru: The CRISPR/Cas9 system is easier...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN...scenarios, you can either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target... paper, for example, we generated hepatocytes to examine the effect of a gene on cholesterol metabolism...

  27. CRISPR in the Clinic

    Type
    Blog Post
    ... J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing. Mol Cancer 21, 78 ...Wu et al. "Correction of a Genetic Disease in Mouse Via Use of CRISPR/Cas9." Cell Stem Cell. 13, 659-662...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field...dominant cataract disorder caused by a single copy of a gene known as Crygc (Wu et al). They found that the cataracts...phenotype could be rescued by zygote co-injection of Cas9 and a single-guide RNA targeting the mutant allele...able to demonstrate functional correction of the gene in clonally expanded organoids. This work, and many...immunotherapy for cancer treatment by traditional gene therapy pipelines (viral), and researchers are currently...

  28. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the Journal of Visualized Experiments (JOVE)....and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines...motivations behind this research.   Using CRISPR/Cas9 for targeted genomic deletions We were inspired ...with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break...approach is to target two exonic sites within a single gene using paired DSBs. This method allows for deletion...regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. J.Biol.Chem....Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions...

  29. CRISPR Guide Update

    Type
    Blog Post
    ...engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives to Cas9. All ...-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress ...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...

  30. Hot Plasmids - February 2022

    Type
    Blog Post
    ...many CRISPR-based genetic screening methods have primarily used Cas9 to knock out genes, researchers at ...between MARCH5 and WSB2 genes using Cas12a’s ability to knock out multiple genes in parallel. Lastly, this...similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple genes within ... and gene activation, is composed of only 529 amino acids, less than half the size of either Cas9 or Cas12a...to induce high levels of gene activation for both reporter and endogenous genes. It is highly specific ...cellular targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation The Penn...10846-1.   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation by: Gabrielle...
Showing: 61 - 90 of 311 results