...doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains...

...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

..., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous...

... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

...transformation efficiency, or more specifically calculated based on the size of and weight of your library...

...used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma...

...transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed...

...Spalteholtz (10).  He used a solution of benzyl alcohol and methyl salicylate which was later modified ...

...proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA...

...enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options...

...18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...

...guide RNA sequence you used. The ICE tool will calculate overall editing efficiency and determine the profiles...

...measured by qPCR.   Physical titer is typically calculated by two popular PCR-based methods: Quantitative...

...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...

... (including DREADDs) Optogenetics Recombinases Calcium Sensors and other Biosensors Controls To look up...

...and determine the topology of the mitochondrial calcium uniporter (Martell et al., 2012). It is important...

...synthetic ribosome binding site (using the online calculator from the Salis group) (Espah Borujeni et al.,...

...clones from that population. You can additionally calculate the minimum number of clones you would have to...

... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like...

...good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor stand...

...two successful mutations in a single cell. We calculated the likelihood of obtaining homo- and heterozygous...

... later steps in this chain (blue) to infer or calculate things about earlier ones. In practice, multiple...

... the different signals in a certain region or calculate a colocalization factor to assess the spatial ...

...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...

...Hartmann, and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991...

...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should...

... that all group leaders must master. While the calculus of these decisions is complex, here are a few ...

...concentration of the known samples is used to calculate the concentration of protein in the experimental...

...optimized to achieve high knock-in efficiency. To calculate the tagging efficiency in the transfected cells...

...The different labs use different algorithms to calculate which would be the best gRNAs. Interestingly, ...

..., et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature...

... Lysine (D123K), lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His...

... phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based...

...light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins....

... Standard Curve?  Amplification Efficacy Calculation Amplification Efficacy Assumptions Standard...

...pLV-EF1-Cre-PGK-Puro Cre EF-1 alpha Lentiviral Alcudia 108639 fli1aCreERT2 Cre-ERT2; endothelial reporter...

...proteinopathy, ALS Nicolas Fawzi 98664 MBP-hnRNPA2_deltaLC HNRNPA2B1 His, MBP T7 Multisystem proteinopathy...

... forget to factor this into your calculations later (see calculation example below). Seed 300,000 cells... a CSV file which will be used to calculate the titer. Calculations In the experimental setup above, the...20 200 40 400 80 800 160 1600 To calculate the titers, first calculate the number of viruses per cellular...glutaGRO, Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...over copies\ RPP30}$$ Use the viruses/genome to calculate the infectious titer: $$T = {V*C*D\over v}$$ Where...titer obtained from the appropriate dilutions to calculate the final infectious titer. Tips and Troubleshooting...green). To increase the accuracy of the titer, calculate an average of several dilutions. Figure 1: ddPCR...

...new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes should... mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...supports normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation...Aliquot 3 mL SOC into each of four 14 mL Vented Falcon Tubes and have 1 mL SOC per electroporation readily...all liquid from cuvette and add to 14 mL vented Falcon Tube containing 3 mL SOC. Repeat for each of the...DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After the...

... of PBS. Calculate the fraction of fluorescent-positive cells in each well. When calculating titer, only...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...remaining well, a cell count is required for calculating the titer. Incubate for 48–72 h. Gently aspirate...leading to underestimation of the true titer. Calculate the transduction units per mL (TU/mL) using either...

...CI) Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...will be used to seed the 96-well plate. Sample Calculation First, use a hemocytometer to quantitate the ...well plate, make 10 mL of a 5 cell/mL solution. Calculate the total cells needed: Total cells needed: 10...

...Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution...by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously by hand for 10...homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used. Shake vigorously...

...Thermo Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed to have a 1:... Therefore, for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1 μg Volume...

... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...

.... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...integration events decreases as the dilution increases. Calculate the transduction units per mL (TU/mL) as follows...

...Addgene. The values highlighted below in red were calculated using this plasmid, but will change if you use...use a different plasmid. Sample Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration...

...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...

...trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart Editor ,...based on your sample’s HC + LC area. Example calculation: If the sample HC + LC Area is 18179.442 and ...