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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 162 results for: ALC

Showing: 101 - 150 of 162 results

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

  5. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ..., which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine ...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains...

  9. Pooled Library Amplifications

    Type
    Blog Post
    ...transformation efficiency, or more specifically calculated based on the size of and weight of your library...

  13. Chemogenetics Guide

    Type
    Guide
    ... phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ...proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like...

  38. Deep Dive: qPCR

    Type
    Blog Post
    ... Standard Curve?  Amplification Efficacy Calculation Amplification Efficacy Assumptions Standard...

  40. Lentivirus ddPCR Titration

    Type
    Protocol
    ... forget to factor this into your calculations later (see calculation example below). Seed 300,000 cells... a CSV file which will be used to calculate the titer. Calculations In the experimental setup above, the...20 200 40 400 80 800 160 1600 To calculate the titers, first calculate the number of viruses per cellular...glutaGRO, Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...over copies\ RPP30}$$ Use the viruses/genome to calculate the infectious titer: $$T = {V*C*D\over v}$$ Where...titer obtained from the appropriate dilutions to calculate the final infectious titer. Tips and Troubleshooting...green). To increase the accuracy of the titer, calculate an average of several dilutions. Figure 1: ddPCR...

  41. CRISPR Library Amplification

    Type
    Protocol
    ...new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes should... mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...supports normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation...Aliquot 3 mL SOC into each of four 14 mL Vented Falcon Tubes and have 1 mL SOC per electroporation readily...all liquid from cuvette and add to 14 mL vented Falcon Tube containing 3 mL SOC. Repeat for each of the...DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After the...

  42. Fluorescence Titering Assay

    Type
    Protocol
    ... of PBS. Calculate the fraction of fluorescent-positive cells in each well. When calculating titer, only...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...remaining well, a cell count is required for calculating the titer. Incubate for 48–72 h. Gently aspirate...leading to underestimation of the true titer. Calculate the transduction units per mL (TU/mL) using either...

  43. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...CI) Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...will be used to seed the 96-well plate. Sample Calculation First, use a hemocytometer to quantitate the ...well plate, make 10 mL of a 5 cell/mL solution. Calculate the total cells needed: Total cells needed: 10...

  44. Kit Free RNA Extraction

    Type
    Protocol
    ...Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution...by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously by hand for 10...homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used. Shake vigorously...

  45. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Thermo Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed to have a 1:... Therefore, for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1 μg Volume...

  46. Centrifugation

    Type
    Protocol
    ... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...

  47. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    .... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

  48. Colony Formation Titering Assay

    Type
    Protocol
    ...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...integration events decreases as the dilution increases. Calculate the transduction units per mL (TU/mL) as follows...

  49. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Addgene. The values highlighted below in red were calculated using this plasmid, but will change if you use...use a different plasmid. Sample Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration...

  50. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...
Showing: 101 - 150 of 162 results