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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 191 results for: CO

Showing: 101 - 150 of 191 results

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    ... independently of any other accessory proteins or co-factors, thus allowing for broad applications in ...

  4. p53 Pathway

    Type
    Collection
    ...binding activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...control over when Cas9 activity is blocked. Hence, co-opting optogenetic techniques allows greater precision... LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein, co-expressed with Cas9 or dCas9 Repression of gene editing...

  9. To Each HIS Own

    Type
    Blog Post
    ...present in most production systems and can bind and co-elute with the protein of interest (Bornhorst 2000...

  15. Adenovirus Guide

    Type
    Guide
    ...into the adenoviral backbone plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy...series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest....

  16. CRISPR in the Clinic

    Type
    Blog Post
    ...the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide RNA targeting...

  18. 6 Tips for Grant Writing

    Type
    Blog Post
    ...contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter what facet...

  24. Brain Armamentarium

    Type
    Collection
    ...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...

  25. Lentivirus Plasmids

    Type
    Collection
    ... EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dtomato version...

  26. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...also activate muscarinic-derived DREADDs. A ligand co-developed by Bryan Roth's and Takafumi Minamimoto's...

  34. CRISPR Guide

    Type
    Collection
    ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

  41. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push forward candidate...

  48. CRISPR Plasmids - Tagging

    Type
    Collection
    ...protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled...

  49. Fluorescence Titering Assay

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...
Showing: 101 - 150 of 191 results