We narrowed to 4 results for: CO

1. Adeno-associated virus (AAV) Guide

   Type

   Guide

   ... packaged in separate rAAV vectors which are then co-delivered to the host cells. The full-length transgene... sequence at its 5' end. When these viral vectors co-infect a cell, they form concatemers and splice together...both sequences present substantial sequence overlap. Co-expression induces homologous recombination and expression...

2. CRISPR Guide

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   Guide

   ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

3. Adenovirus Guide

   Type

   Guide

   ...into the adenoviral backbone plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy...series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest....

4. Gamma-Retroviral Vector Guide

   Type

   Guide

   ...described above (envelope, packaging, and transfer) are co-transfected into the HEK293T packaging cell line....