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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10 results for: CO

Showing: 1 - 10 of 10 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... tissue culture plate. Incubate cells at 37°C, 5% CO 2 overnight. Although cells should regularly be passaged...cells from the plate. i. Incubate cells at 37°C, 5% CO 2 for 12-15 hours. Day 3: j. In the morning, change... transfected cells. k. Incubate cells at 37°C, 5% CO 2 for 24 hours. Day 4: l. Harvest media from cells...antibiotics to the cells and incubate at 37°C, 5% CO 2 for 24 hours. Day 5: n. Harvest media from cells...target cells in ten 6 cm plates and grow at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be...1: a. Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should be approximately...your target cell line. d. Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours...

  2. General Transfection

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Gently aspirate media, add 10 mL fresh...

  3. Lentivirus Production

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection...

  4. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm ... in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm....

  5. Fluorescence Titering Assay

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

  6. Colony Formation Titering Assay

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ... pipette Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container...
Showing: 1 - 10 of 10 results