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We narrowed to 20 results for: CO

Showing: 1 - 20 of 20 results

  1. Worm Expression Resources

    Type
    Collection
    ...Link opens in a new window) Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing...Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

  2. CRISPR References and Information

    Type
    Collection
    ...neuronal co-injection marker); pCFJ104 (body wall muscle co-injection marker); pCFJ90 (pharyngeal co-injection...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature ...human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar...

  6. Brain Initiative Collection

    Type
    Collection
    ...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR...

  7. E11 Bio PRISM Collection

    Type
    Collection
    ...plasmids can be packaged as AAVs and pooled together for co-delivery. Alternatively, plasmids can be combined...

  8. Rett Syndrome

    Type
    Collection
    ...ability to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests...D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and...

  9. Antibody Production

    Type
    Collection
    ... recombinant antibody preps contain low levels of co-purified plasmid from transfection. This residual...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-...

  11. TALEN Guide

    Type
    Collection
    ... ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr. Voytas...

  12. p53 Pathway

    Type
    Collection
    ...binding activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of...

  13. CRISPR Guide

    Type
    Collection
    ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled...

  15. Lentivirus Plasmids

    Type
    Collection
    ... EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid ...Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875...

  17. Brain Armamentarium

    Type
    Collection
    ...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a 140068 Anti-Kv1.6...2b] GluN2C/NR2C Rat Mouse IgG2a 222145 Co-Rest/RCOR1 [K72/2R] Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin...into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs are secreted into the...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...molecule presenting its cognate antigen along with co-stimulation activates the T cell, whereas T cell ...anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. TGFbeta...anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. Symbol... HRAS1, K-RAS, N-RAS, RASH1 ICOS inducible T-cell co-stimulator AILIM, CD278, MGC39850 IFNG interferon...
Showing: 1 - 20 of 20 results