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Addgene

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We narrowed to 139 results for: MAX

Showing: 101 - 139 of 139 results

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...in some cases, over 20-fold higher editing than PEmax. The authors hypothesized that the improved efficiency...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    ...large edits with similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    ... been used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

  13. March for Science

    Type
    Blog Post
    ...Science Part: Democratic values and their role in maximizing the objectivity of science.  Scientific American...

  17. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...quantities of DNA, you’ll need to do a midiprep or a maxiprep. In these purifications, you generally lyse the...

  25. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...tract lengths seem much shorter. Q10: What is the maximum amount of DNA that can be inserted into the genome...

  27. DNA Quantification

    Type
    Protocol
    ...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is ...and most have a specific wavelength that they maximally absorb. By measuring the absorbance of a liquid...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally...

  28. Adenovirus Guide

    Type
    Guide
    ...use of pAdEasy-1. Created with BioRender.com. AdMax™ AdMax™ is another well used rAdV vector production...adenoviral genes necessary for packaging. In the AdMax™ system, recombination between both plasmids occurs...the HEK293 packaging cell line, engineered for maximum adenoviral production, and is driven by use of ...

  29. CRISPR Guide

    Type
    Guide
    ... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

  30. Pouring LB Agar Plates

    Type
    Protocol
    ...and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will be screening...like to make 20 plates, and our plates can hold a maximum of 10 mL, we’ll want 200 mL of media total. Importantly...

  31. Guide to Using Pooled Libraries

    Type
    Guide
    ...either case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library ...process. Amplify the library (electroporation and maxiprep). If delivering as virus, make virus; this creates...

  33. Water Bath Protocol

    Type
    Protocol
    ...fill the water bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside...

  35. Pipetting Protocol

    Type
    Protocol
    ... might hold a pipette. Without moving past the maximum possible setting on the pipette, set the desired...

  37. AAV Production in HEK293 Cells

    Type
    Protocol
    ...but do not stick to PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM...

  38. Plan Your Experiment

    Type
    Guide
    ... it is also important to consider factors that maximize cleavage of the desired target sequence or on-target...
Showing: 101 - 139 of 139 results