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We narrowed to 273 results for: aav

Showing: 101 - 150 of 273 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Gether lab, now available as ready-to-use AAV vectors.   ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    ...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed...

  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ...

  10. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider...

  11. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once...

  13. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research... excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of...

  14. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned...

  15. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...

  16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

  17. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request  ...lab has a limited quantity of AAVs that can be requested by contacting Alexander Lohman ([email protected]... T7 pAAV-hSYN1-NES-OCaMP-WPRE pAAV hSyn pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE pAAV Ef1a ... pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our...

  21. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post...

  23. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver...

  24. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print...

  25. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral...

  28. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... it can be packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter... smaller than SaCas9 and is also compatible with AAV delivery. NmCas9, another small ortholog, displays... Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page Resources...

  29. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors New AAV tools to selectively...from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

  30. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that...

  31. How Addgene Creates Content

    Type
    Blog Post
    ...Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data Hub...enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to easily...

  33. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...and shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one...my lab mate’s with her project. Unfortunately the AAV didn’t give us the results we hoped for, but it did...

  34. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce...

  35. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic...

  36. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively...

  38. Addgene: The First Twenty Years

    Type
    Blog Post
    ...vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing supportive...be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

  40. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging ...rh10 capsid James M. Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2... Virus (AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids...Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated...immunological effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should...to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...

  43. Brain Armamentarium

    Type
    Collection
    ...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell Viviana Gradinaru 213941-AAV1 pAAV_BiPVe3_ChR2_mCherry AAV construct...Alias: pAAV_WDC0004_GCaMP6f Gordon Fishell Viviana Gradinaru 213937-AAV1 pAAV_BiPVe4_ChR2_mCherry AAV construct...Alias: pAAV_WDL0003_jGCaMP8m Gordon Fishell James M. Wilson 213917-AAV1 pAAV_BiLAMP5e3_GCaMP6f AAV construct...Alias: pAAV_YWE4.2_GCaMP6f Gordon Fishell Viviana Gradinaru 213856-AAV1 pAAV_BiVIPe4_ChR2_mCherry AAV construct...Alias: pAAV_S9E10_GCaMP6f Gordon Fishell Viviana Gradinaru 213815-AAV1 pAAV_BiSSTe10_ChR2_mCherry AAV construct...Science , Bosiljka Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier...enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct...

  44. Retrograde AAV viral preps

    Type
    Collection
    ...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-...Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE Project 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

  45. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_...Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown...

  46. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm...in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids...

  48. Recombinases AAV Preps

    Type
    Collection
    ...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...William Pu 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T...

  49. Brain Initiative Collection

    Type
    Collection
    ...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...

  50. Biosensor AAV Preps

    Type
    Collection
    ...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE 167572 AAV-hSyn-Ribo-jGCaMP8s...176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-...
Showing: 101 - 150 of 273 results