We narrowed to 272 results for: aav

...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

...Micropeptide killswitch for biomolecular condensates AAV-CRISPRa-engineered fat cells fight cancer Frugal ...interactions. Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies.... https://doi.org/10.1038/s41586-025-09141-5   AAV-engineered adipocytes suppress tumor progression ...normally expressed in BAT. When donor-derived CRISPRa-AAV-treated WAT cells were transplanted into the vicinity...experiments. B) Constructs for tet-inducible CRISPRa-AAV engineering. Adapted from Nguyen et al. (2025) under...cell and gene therapy, so please check it out! Find AAV-CRISPRa plasmids here! Nguyen, H. P., et al. (2025...microenvironment (Nguyen et al., 2025). The team used AAV9 to deliver CRISPRa components to adipose cells to...

...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Gether lab, now available as ready-to-use AAV vectors.   ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether...

...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...

... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ...

...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ...

...restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila...

...project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed...

...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider...

...Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once...

...adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs ...associated with AAV; co-infection with both constructs produces a minimum of 8 colors. The use of AAV provides...

...a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research... excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of...

...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned...

...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...

...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

...lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to create both transient cell...You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations...

...Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request  ...lab has a limited quantity of AAVs that can be requested by contacting Alexander Lohman ([email protected]... T7 pAAV-hSYN1-NES-OCaMP-WPRE pAAV hSyn pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE pAAV Ef1a ... pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab...

...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in...

...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our...

...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post...

...cells, commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics...

...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver...

...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print...

... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ...

...are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral...

...producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second edition includes...

... it can be packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter... smaller than SaCas9 and is also compatible with AAV delivery. NmCas9, another small ortholog, displays... Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page Resources...

...available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors New AAV tools to selectively...from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that...

...Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data Hub...enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to easily...

...Adeno-Associated Viral (AAV) Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org Adeno-Associated Viral (AAV) Vector Guide...Transfection AAV Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration...AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging ...rh10 capsid James M. Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2...Plasmids AAV Helper Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material...These genes are not present in the AAV genome, but are required for AAV viral replication. This collection...

...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell Viviana Gradinaru 213941-AAV1 pAAV_BiPVe3_ChR2_mCherry AAV construct...Alias: pAAV_WDC0004_GCaMP6f Gordon Fishell Viviana Gradinaru 213937-AAV1 pAAV_BiPVe4_ChR2_mCherry AAV construct...Alias: pAAV_WDL0003_jGCaMP8m Gordon Fishell James M. Wilson 213917-AAV1 pAAV_BiLAMP5e3_GCaMP6f AAV construct...Alias: pAAV_YWE4.2_GCaMP6f Gordon Fishell Viviana Gradinaru 213856-AAV1 pAAV_BiVIPe4_ChR2_mCherry AAV construct...Alias: pAAV_S9E27_GCaMP6f Gordon Fishell James M. Wilson 213833-AAV5 pAAV_BiCHATe27_GCaMP6f AAV construct...Alias: pAAV_S9E10_GCaMP6f Gordon Fishell Viviana Gradinaru 213815-AAV1 pAAV_BiSSTe10_ChR2_mCherry AAV construct...Science , Bosiljka Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier...

...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-...Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE Project 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...

...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40... 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley Thomas Carmichael 214848 AiP13780: pAAV-AiE0743m...

...then easy to package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. ...acceptor and two putative splice enhancers. After AAV-mediated delivery to iPS-derived cortical neurons...

...and shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one...my lab mate’s with her project. Unfortunately the AAV didn’t give us the results we hoped for, but it did...

...Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce...

...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode ...for serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde. Viral...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid... Cart AAV9 37825-AAV9.T 20 µL $ 150 Add to Cart AAV11 37825-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde...150 Add to Cart AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 150 Add...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description...

...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 5, 9, rg* GENIE Project 162379 pGP-AAV-syn-FLEX-jGCaMP8f-WPRE...169258 AAV-hSyn-Soma-jGCaMP8f Syn soma-jGCaMP8f none Constitutive 1, 9, rg* Marianne Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE...176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Loren Looger 176756 AAV-EF1a-jGCaMP8f-WPRE...pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE Project 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE Project 169256 AAV-hSyn-Soma-jGCaMP8s...176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 9, rg* Loren Looger 176752 AAV-CamKIIa-jGCaMP8s-WPRE...

...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

...proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic...

...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively...

...Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful...Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech... in one of seven serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11, or AAVrg). For more information, visit...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro...protection levels. High quality and high titer in-stock AAV preps are affordable, available in small volumes,...

...vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing supportive...be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

...the protein of interest. In our studies, we used AAV viral vectors to express hM4Di in neurons of rhesus...years ago (Field et al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys...

...and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang et al. found that Cre-DOG could ...optogenetic studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system...

... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ... # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles...production methods and quality control for lentivirus and AAV. Viral...Viral Production Jump To: Viral Production Overview AAV Production Titer Quality Control Resources Lentivirus...