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Addgene

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We narrowed to 435 results for: {{{s}}}

Showing: 41 - 60 of 435 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin JF, Ansorge...Link opens in a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed...Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth...Ramakrishnan C, Fenno L, Deisseroth K, Herry C, Arber S, Lüthi A. 2016. Midbrain circuits for defensive behaviour...

  2. An Introduction to Adenovirus

    Type
    Blog Post
    ...., Bull, R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan...Reyes-Sandoval, A., Harris-McCoy, K. C., Hensley, S. E., Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre...Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W. S. (1996). The adenovirus death protein...Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https://doi.org/10.1073/PNAS.95.19.11377.../JVI.70.4.2296-2306.1996. PMID: 8642656. Wold, W. S. M., & Toth, K. (2013). Adenovirus Vectors for Gene...

  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9... TadA and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess the effect...created ABE8 constructs using a catalytically “dead” S. pyogenes Cas9 (dC9-ABE8.x-m/d). The dC9-ABE8.x-m/...scope of adenine base editors The most commonly used S. pyogenes Cas9 requires the target site in the genomic...-Cas9 (PAM: NG) to create NG-ABE8.xm/d and the S. aureus Cas9 (PAM: NNGRRT) to create Sa-ABE8.x-m/d. With...NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double.... https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted...Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin...Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA, Ramsden DA (...

  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9 stability in human plasma ...SpCas9 can tolerate. Harrington et al tested GeoCas9’s love of heat in two ways. The first set of experiments... mammalian cells. Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9...organisms at lower than optimal temperatures. GeoCas9’s thermostability could also be used with in vitro molecular...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert...domains derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their...Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Enhanced prime.../10.1038/s41587-021-01025-z Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph...Raguram, A., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar, J., Osborn, M. J., .../doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin, S., Zong, Y., Yu, H., Zhu, Z., Liu, G., Kou, L., Wang...021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V....

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent...oligomerisation. Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP. FEBS Lett. 2017 Dan Mulvihill mVenus...visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared ...protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol....

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ....1101/2023.03.20.533580 Goodwin, M. S., Croft, C. L., Futch, H. S., Ryu, D., Ceballos-Diaz, C., Liu, X...Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing...producer cells for further processing. What component(s) you need to collect will depend on the serotype, ....1089/hum.2020.120 Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... B.-Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K.D., Lindo, S., Michael, S., Kuleshova, E., Ojala...complications. References Aschauer, D.F., Kreuz, S., and Rumpel, S. (2013). Analysis of transduction efficiency...Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local...PubMed Central PMCID: PMC2293646. Jin, J., and Maren, S. (2015). Prefrontal-Hippocampal Interactions in Memory...Mise, N., Obata, Y., Wickersham, I.R., and Tonegawa S. (2014). Cell type-specific genetic and optogenetic...22196330. PubMed Central PMCID: PMC3275419. Maday, S., Twelvetrees, A.E., Moughamian, A.J., and Holzbaur...Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., Mortrud...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ..., Valois, E., Solley, S. C., Braig, F., Lach, R. S., Baxter, N. J., Kosik, K. S., Arias, C., Acosta-Alvear...coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing) technique... amplification kit. In this case, regions of gene S and Orf1ab gene are amplified. RPA reactions also ...A. H., Sinha, D., Sharma, N., Kumar, M., … Maiti, S. (2020). Rapid, field-deployable nucleobase detection...-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY ...2020.04.20.052159 Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using CRISPR...

  11. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM.... coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo genome editing with a ...doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune system cleaves...019-0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...doi.org/10.1038/nature14299  Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, Börner K, Grimm...doi.org/10.1002/biot.201400046  Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...deliver the appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular cell...Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der...https://doi.org/10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...

  14. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression in Peripheral... JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, ...Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power of the Lock, the Weakness..., Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research and ...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...wondered if it could overcome some of Cas9’s shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from...nucleotide 5’ overhang 18-23 bases away from the PAM. Cpf1’s staggered cleavage pattern opens up the possibility...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates...with SpCas9. Kleinstiver et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity...Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin ...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann,...https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells.../doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston...RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., Fotouhi, M., Jones, C., Ayoubi, R., ... M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization...10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, ..., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., & Roach, K. M. (2021). TGFβ1 induces...

  17. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts ...protein, which they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable... cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide tag forming a rapid...bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., &...Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert...Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Enhanced prime....2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V....

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... B. S., Purohit, A. M., Grippo, R. M., Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants...andrewgyork.github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical...Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical...doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule...

  20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...K., Kim, Y., Lee, S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019... Kim, H., Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent..., Schep, R., Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer... Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G., Peric-Hupkes, D., van den Berg, J., Beijersbergen...
Showing: 41 - 60 of 435 results