...molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73 on specific solid as ...cell-surface antigen production to evade the immune system. 3. Escape - Tumor cells that survive this phase are ... situ before being injected back into the body in 3 doses intravenously. B cells and T cells activated...medicine 10.9 (2004): 942-949. PubMed PMID: 15322536. 3. Dunn, Gavin P., et al. "Cancer immunoediting: from...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at Binghamton University. She works ...

... 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion proteins. One of the...potential to form large stable polymers of dimers. 3) FRET biosensors that contain two dimerizing FPs can...localize incorrectly and may function improperly. 3. Incorporation into a FRET biosensor A FRET biosensor...DsRed or high affinity FPs such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs...2002. 296(5569): p. 913-6. PubMed PMID: 11988576. 3. Matz, M.V., et al., Fluorescent proteins from nonbioluminescent...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...

...primer sequences! Primers consist of two regions: the 3’ end binds template DNA for PCR amplification, while...vector sequence between modification sites (see Figure 3), producing multiple fragments with specific homologous...introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple...not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix. When PCR is not...PubMed PMID: 8346047. PubMed Central PMCID: PMC331480. 3. García-Nafría, Javier, Jake F. Watson, and Ingo H...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

...in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout/knock-in...Figure 1, large sequences corresponding to exons 1 and 3 of the targeted gene have been cloned into the vector...Neomycin resistance (NeoR) gene between exon 1 and 3 of the targeted gene. Upon recombination, the GFP/...resistance marker using Cre recombinase. Since there are 3 loxP sites, recombination can occur in multiple ways...

...podcast from producers Tyler Ford and Eddy Page. 2:00 - 3:55 : Alex introduces himself and explains why he decided...decided to do an MD PhD - it's all in the family. 3:56 - 7:04 : Alex describes the MD PhD process and ...Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church and Collins labs at the...

...delivery." Elife 3 (2015): e04766. PubMed PMID: 25497837. PubMed Central PMCID: PMC4383097. 3. Kim, Sojung...contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 ... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

...abundant incorporation of cysteine in their proteomes (3). This led to the eventual discovery of a naturally...which can be specifically recognized by the Sep-tRNA. 3. An expression plasmid encoding the ORF to be expressed...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...blogger Natalie Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research...

...terms of communicating ideas. In 2012, for example, 3 billion hours of video were watched each month, in...WeShareScience is a free platform for sharing short, 3 to 5 minute videos about research. Videos can be shared...means for communicating research. Short videos of 3 to 5 minutes provide more than enough time for researchers...by the numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science...Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the...

...break membranes outside of their natural context or 3) be modified to extend at different pH levels. Domesticating...Protistology 48, 290–296 (2012). PubMed PMID: 22356923. 3. Polka, J. K. & Silver, P. A. A Tunable Protein Piston...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department...

...“blind” to this very important difference (Figure 3). If you are interested in harnessing the power ... PubMed PMID: 27089539. PubMed PMCID: PMC4835065. 3. Mock, U., Hauber, I. & Fehse, B. Digital PCR to assess...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

... The detection threshold for these RNA sequences (3 nM), however, was quite high and above what would ... samples at clinically relevant concentrations (1-3 fM; 1x106-fold lower than before), and our combined...PMID: 25417166. PubMed Central PMCID: PMC4265554. 3. Pardee, Keith, et al. "Paper-based synthetic gene... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...

...sequence only functions at the extreme C-terminus [3]. You must therefore determine your cloning strategy...PMID: 19819147. Pubmed Central PMCID: PMC2784028. 3. Munro, Sean, and H. R. Pelham. "Use of peptide tagging...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... from Albert Einstein College of Medicine. Her doctoral research was conducted in the laboratory of Erik...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

...degradation of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small...with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature Chemical Biology for...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

... strand break), increasing editing efficiencies 2-3-fold. However, the process of double nicking increases...degradation by Cas9 binding, but pegRNAs have an extended 3′ tail containing the reverse transcriptase template...but it cannot drive prime editing. To protect the 3′ end, the Liu lab appended RNA pseudoknots, calling...expressed in eukaryotes, binds and stabilizes the 3′ tail of pegRNAs (Yan et al., 2024). The team fused...for certain edits           ~   3′ RNA pseudoknot (epegRNA) Improve pegRNA stability...prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...

...PMID: 18295576. PubMed Central PMCID: PMC4142810. 3. Mitalipov, S. and D. Wolf, Totipotency, pluripotency...potential of muscle stem cells. Dev Cell, 2001. 1(3): p. 333-42. PubMed PMID: 11702945. 6. Cowan, C.A.... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

...understand your proposal to give you proper consideration. 3. Present an easy to follow seminar See item 2. Attendees...? How will your approach resolve this problem? (2-3 sentences) What will be the focus of your first... potential collaborations, critiques of your postdoctoral research, etc. The second question is whether...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

... it in. I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge ...this job specifically looked for a researcher with 3 years+ of cancer research experience, a PhD or a Master...

...into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate, for Stage 3, that the... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

...evidence you need. For example, as shown in this study [3], hippocampal neurons are activated when the mouse...PMID: 25242321. Pubmed Central PMCID: PMC4181379. 3. Kheirbek, Mazen A., et al. "Differential control ...retrieval strategies for remote memories. Cell. 2011;147(3):678-89. Pubmed PMID: 22179551. Pubmed Central PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...228, no. 2 (2012): 267-75. PubMed PMID: 22767332.  3. Kim, Dohoon, Chun-Hyung Kim, Jung-Il Moon, Young-...PubMed PMID: 19337237. PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak... deriving human iPS cells." Nature Methods 7, no. 3 (2010): 197-99. PubMed PMID: 20139967. PubMed Central...a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles...expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus...plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if...