5 Tips for Troubleshooting Viral Transductions
Type
Blog Post
... Leila Haery...
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Tips for Titering Your Lentiviral Preps
TypeBlog Post...4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral vector... -
Rabies and Neuronal Tracing
TypeBlog Post... Leila Haery... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...References Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work for every plasmid... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post....2005.06.003 Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...should be able to keep cells moist and hopefully aerated with a thin layer of molecular biology-grade agarose... -
10 Basic tips for mammalian cell culture
TypeBlog Post...talking because talking generates microbe-laden aerosols that may enter the hood. Planning your experiments... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan JS (2020) ... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...independently of oxygen, making suitble for use in anaerobic conditions. Maturation Time: Maturation time is... -
Brain Initiative Collection
TypeCollection... strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation...BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-... -
MXS Chaining
TypeBlog Post... Leila Haery... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post... making your virus dilutions. This will reduce aerosols that could potentially lead to contamination. ... -
Plasmids 101: Codon usage bias
TypeBlog Post...22.7 (2004): 346-353. PubMed PMID: 15245907. 5. Maertens, Barbara, et al. "Gene optimization mechanisms... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post... Leila Haery... -
March for Science
TypeBlog Post...proposing to defund aspects of research at National Aeronautics and Space Agency, the Environmental Protection... -
Five Popular Model Organisms, Part 2
TypeBlog Post...obtainable growing conditions. As a facultative aerobe, it can thrive with or without oxygen. Additionally... -
Cre-lox system
TypeCollection...neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 15509 pBS-M71-IRES-CreFNF M71 targeting vector...neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...agar plate before growing in liquid culture. More aeration may help to increase the density of the culture... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... Leila Haery...