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Showing: 1 - 20 of 3030 results

  2. pCSZPKIamutHAERT(#119)

    Plasmid
    #184076
    Purpose
    cyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfmutPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…
    Promoter
    sCMV+SP6
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184076-plasmid-map-sequence-id-365868

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  3. pCSZPKIaHAERT(#118)

    Plasmid
    #184075
    Purpose
    cyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184075-plasmid-map-sequence-id-365717

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  4. pT2iD6Hoxa13aER4(#685)

    Plasmid
    #184100
    Purpose
    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfHoxa13a-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184100-plasmid-map-sequence-id-364020

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  9. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154846-plasmid-map-sequence-id-348286

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  11. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Available since
    Aug. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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  13. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  15. pMU131

    Plasmid
    #66811
    Purpose
    Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI
    Depositor
    Lee Lynd
    Article
    Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66811-plasmid-map-sequence-id-117172

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Showing: 1 - 20 of 3030 results