...Actinomyces naeslundii, A. viscosus, Aerobacter aerogenes, Aeromonas hydrophila, Agrobacterium tumefaciens... Achromobacter parvulus, Acinetobacter spp., Aeromonas spp., Agrobacterium spp., Alcaligenes spp., Aliivibrio...Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, Methylophilus methylotrophus, ...Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, ...Gram-negative  Acinetobacter calcoaceticus, Aeromonas liquefaciens, A. salmonicida, Agrobacterium tumefaciens...meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas...

... properties Facultative anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes ...non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic yeast, meaning it can...

...applications that require anaerobic conditions. In experiments requiring anaerobic conditions, proteins that...consequence GFP remains non-fluorescent under anaerobic conditions. This holds true for any of the AvGFP...works best for a specific biological system under anaerobic conditions needs to be examined on a case-by-case...

...assurance of the serum being contamination free. The aerosols that are generated (while talking or pipetting...among the leading causes of contamination. These aerosols generated will enter the culture medium during...cell line remains exposed for a longer time. The aerosols are invisible to naked eyes and hence cannot be...also solve the problem of contamination through aerosols. Perform culturing without the use of antibiotics...

...trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens...disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria isolated...

...sound credible and cool when you casually mention “Haery et al., showed that MYC expression was increased... better than perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore.... Leila Haery...

...vector information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics... Leila Haery...

...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...eArch3.0, eArchT3.0 Browse Archaerhodopsin plasmids . Wild-Type Mac Leptosphaeria rhodopsin (Mac) is a blue-green...Kir2.1 566 Leptosphaeria rhodopsins Mac Light-activated outward proton pump from Leptosphaeria maculans ...thus expressed in all GABAergic neurons. In this case, the subpopulation of GABAergic neurons being activated...light-activated proton pump derived from the fungus Leptosphaeria maculans . Mac and its variants allow for inhibition...photocurrent amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants...signal provide improved membrane targeting 589 Archaerhodopsins Arch Light-activated outward proton pump from...

...human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a...expression of your gene of interest. References Haery L, Deverman BE, Matho KS, Cetin A, Woodard K, Cepko...

...example, the mDlx enhancer restricts expression to GABAergic interneurons. These vectors can also be used as... Leila Haery...

...The Dlx element directs expression in forebrain GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry... Leila Haery...

...during production), there is a significant risk for aerosol contamination. As most centrifugation has to be... and other BSL-2 materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation...

...learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade et al., 2016), video, educational...101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard...

...toxin genes isolated from E. coli or Psuedomonas aeruginosa. These toxins were a mix of Type VI secreted ...et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange." Nature...

...Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene Confirmed Speakers Constance...Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be speaking or leading workshops...Woodard 4:00 PM Wrap Up & Next Steps Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers...and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, Cetin A, Woodard K, Cepko...

...architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing by NHEJ and HDR. Can...Cas9 Richter et al., 2016 tsRC9 RsLOV from R. sphaeroides Cas9, dCas9 Gene editing Zhou et al., 2018 ...

... requires shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath...the door. BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded and unloaded...

...opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that can actively...

...BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-... strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation...

...Phosphoproteins Using Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ...release factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Söll...