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  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Actinomyces naeslundii, A. viscosus, Aerobacter aerogenes, Aeromonas hydrophila, Agrobacterium tumefaciens... Achromobacter parvulus, Acinetobacter spp., Aeromonas spp., Agrobacterium spp., Alcaligenes spp., Aliivibrio...Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, Methylophilus methylotrophus, ...Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, ...Gram-negative  Acinetobacter calcoaceticus, Aeromonas liquefaciens, A. salmonicida, Agrobacterium tumefaciens...meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas...
  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ... properties Facultative anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes ...non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic yeast, meaning it can...
  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...applications that require anaerobic conditions. In experiments requiring anaerobic conditions, proteins that...consequence GFP remains non-fluorescent under anaerobic conditions. This holds true for any of the AvGFP...works best for a specific biological system under anaerobic conditions needs to be examined on a case-by-case...
  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...assurance of the serum being contamination free. The aerosols that are generated (while talking or pipetting...among the leading causes of contamination. These aerosols generated will enter the culture medium during...cell line remains exposed for a longer time. The aerosols are invisible to naked eyes and hence cannot be...also solve the problem of contamination through aerosols. Perform culturing without the use of antibiotics...
  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens...disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria isolated...
  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...sound credible and cool when you casually mention “Haery et al., showed that MYC expression was increased... better than perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore.... Leila Haery...
  7. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...vector information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics... Leila Haery...
  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a...expression of your gene of interest. References Haery L, Deverman BE, Matho KS, Cetin A, Woodard K, Cepko...
  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...example, the mDlx enhancer restricts expression to GABAergic interneurons. These vectors can also be used as... Leila Haery...
  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...during production), there is a significant risk for aerosol contamination. As most centrifugation has to be... and other BSL-2 materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation...
  11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade et al., 2016), video, educational...101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard...
  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...toxin genes isolated from E. coli or Psuedomonas aeruginosa. These toxins were a mix of Type VI secreted ...et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange." Nature...
  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing by NHEJ and HDR. Can...Cas9 Richter et al., 2016 tsRC9 RsLOV from R. sphaeroides Cas9, dCas9 Gene editing Zhou et al., 2018 ...
  14. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...lab-oriented environmental efforts check out Leila Haery’s blog series, “Bringing Sustainable Practices to...
  15. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... He works with gene expression in Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional...
Showing: 1 - 20 of 56 results