We narrowed to 1,092 results for: ANT;

... protein component of MMR) mutant to inhibit MMR PE5 — PE3 plus MLH1 mutant to inhibit MMR PEmax — optimized...QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg Plant OsU3 pegRNA ...Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources Prime editing is a “search... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia Gao 172716 ...

...single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast ... a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9...for easy visualization via direct fluorescence. Samantha Morris 115644 Morris CellTag Barcode Library Version...for easy visualization via direct fluorescence. Samantha Morris 115645 Morris CellTag Barcode Library Version...for easy visualization via direct fluorescence. Samantha Morris Control Lentivirus Browse our list of available... express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3)...Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular...Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina. Mol Ther . Feb...mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods...in Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin...Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials...Neuroscience Antibodies and Grow From There Addgene’s antibody resource is funded by a generous grant from the...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production ...can participate: If you make recombinant antibodies or other recombinant affinity reagents, share your...launch of our latest community resource: Recombinant Antibodies! In addition to plasmids and viral vectors...ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing...

...version of the antigen and compare staining of the antibody to that of the anti-tag antibody; and immunoprecipitation-mass...mean that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform...developed, antibodies rarely work for every assay. Antibodies that are generated against a linear antigen tend...close look at the antigen or antigenic fragment that was used to develop the antibody. Is this protein ...questions on antibody validation? Check out Antibodies 101: Validation!  Download our antibody selection ...experiments require antibodies. You run a quick internet search for the required antibodies and find….hundreds...provide you with some advice on the antibody selection process. Antibodies are not one size fits all When ...

...maximum excitation wavelength (nm), QY D : Donor quantum yield, λ Aem : Acceptor maximum emission wavelength...exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster radius (nm, distance at which...linkers of varying length Evers et al. (2006) Quantitative understanding of the energy transfer between...FRET efficiency standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative controls...evolution of cyan fluorescent proteins towards a quantum yield of 93% Positive and negative controls and...FRET pairs Gadella et al. (2023) mScarlet3: a brilliant and fast-maturing red fluorescent protein Variety...

...Mammalian Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair...created by wild-type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...

...through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1... you know that we don’t expect to produce our guaranteed titer, and will give you the option to proceed...proceed with your order, or not, without a guaranteed titer. Any titer expectations communicated by email ...email will supersede the general guarantees above. We will have our expert viral vector production team carefully...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs...

...European Vector Architecture Open Plant (Link opens in a new window) — Plant SynBio resource Synthetic Biology...Mukhopadhyay Richard Murray Erin O'Shea Martin Parniske Antonio Richart Herbert Sauro Claudia Schmidt-Dannert Pamela... Organism Bacterial Yeast Fungal Worm Mammalian Plant Algal Featured SynBio Deposits Alper Lab Y. Lipolytica...FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit ...Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The...The antibodies distributed through NABOR are recombinant antibodies expressed from plasmids that have ...functional recombinant monoclonal antibody (R-mAb) expression plasmids, allowing for important reagents ...Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies 101: Introduction...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based...preserve and share their own collections. Why Recombinant Antibodies?  One of the benefits of using a plasmid...

..., like developing recombinant antibodies, antibody conjugation, chimeric antibodies, and smaller engineered... IPI Antibody Collection Antibody Guide Addgene Antibody Collection Addgene Antibody Production Antibody...conclusions. Antibody engineering also benefits antibody-based therapeutics. Whether antibodies are used ...regions of your antibody. Gene shuffling Perhaps you like bits and pieces of an antibody but want to know if...whether it's tweaking the antibody-antigen interface or fine-tuning the regions you want to evolve. With this...display are common. ELISA enables quantitative measurement of antigen-antibody interactions in a plate-based...large-scale recombinant antibody production platform. IPI is a leading expert on antibody discovery and...

...as a Grant Professional to mentor and coach you. There are many grant consultants who offer grant training...Associates, Inc., a national grant consulting firm, and Smartegrants, a grant professional development firm... the field of grants. What is a grant professional?  In the past 5-10 years, the Grants Profession has...readiness, identify grant opportunities, secure grant awards, and manage grant projects and funds. You will... for grants, the amount of grant funds available and the increasing regulatory process for grants has ...become a grant professional? Employment opportunities for those with expertise in grant writing, grant management...re seeking a full-time job or want to be an independent grant consultant, building your area of expertise...

... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly Kits...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...leads to slow bacterial growth, or maybe you only want to study the effects of protein expression in the...the stationary phase. In these cases, you may want to control protein levels or to turn on expression ...presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems also use... or introducing specific mutations can reveal important insights into gene function. These genetic changes...

...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...turning on another. The FLEx switch system takes advantage of the orientation specificity of Cre and the ...different types of target sites available, both mutant and wild type. The ability to manipulate the number... to genomic instability and cell death. It is important to incorporate appropriate controls such as Cre-only...

... by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA variant inactivated... genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal... is then recruited to the ribosome, where its anticodon can bind with the complementary codon on the mRNA...aaRS M. jannaschii sulfotyrosine (sY) Bacterial, Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine...inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS/tRNA pair instead. For directed...knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress the TAG nonsense codon). Expresses...

...DNA concentration. It’s important to consider the advantages and disadvantages of each method and when...using UV absorbance to quantify DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent...electrophoresis Quantifying DNA using capillary electrophoresis is similar to quantifying DNA using gel ...fragments are quantified first before larger fragments. Figure 1: The quantification of a RNA ladder...Some last reminders about quantifying DNA In choosing your DNA quantification method there’s many things...UV absorbance is one of the most common ways to quantify DNA. This method involves measuring the absorbance...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally...

...CRISPR systems, the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems...CRISPR Plasmids: Cascade-Cas3 Mammalian Bacteria Plant Resources Cas3 is a unique member of the CRISPR ...double-strand break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound...Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return...

...Edit CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...