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Showing: 41 - 60 of 1087 results

  1. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ...Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta...magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled with anti-GABA-AR β1 (white...white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green... secondary antibodies will bind to each primary antibody.      Look for primary antibodies that have ... combination of antibodies:   Target (Rat) Primary antibody Secondary antibody SCN1A sodium...channels anti-pan-Nav R-mAb K58/35R (Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb ... Goat anti-mouse IgG1 Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG...

  2. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies 101: IsotypesAntibodies 101: Chimeric...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding ... affect antibody applications like ELISAs or Western blots, they are key in helping antibodies drive an...responses? We got you! You can also review antibodies in our Antibodies 101 animation.)  Many types of immune...receptors that bind to the Fc domain of an antigen-bound antibody and induce (or, in the case of the Fc receptors...a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity... et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen...

  3. Antibodies 101: Epitope Tags

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    Blog Post
    ...Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody protocols Additional resources ...of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that works for your... common tags, the antibodies used to detect them, and how they can fix your antibody woes.   What are...without the need for an antibody. Tags can enable the separation of detection of a mutant protein vs. endogenous...monoclonal antibodies. Below we review the most commonly available tags and their antibodies along with...Addgene blog Antibodies 101: Epitope availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men...Epitope tags eliminate the need for target-specific antibodies and have been widely used across species and ...

  4. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...library to a significant degree using different types of AAV. Expression Another advantage of AAV vectors...ones. In those cases, AAV vector screens may be AAVantageous!       In vivo, in vitro, ex vivo CRISPR screens...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E., Kuhn, M., Farouni, R., Lagler...

  5. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...animation is here to help! "Antibodies 101: Antibodies in the Lab" shows how antibodies are used to study proteins... Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...introduction to — or quick refresher on — these common antibody applications.  Thanks for watching, and we hope...

  6. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...which antibody to request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub...Updated AAV Data Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...the Addgene antibody collection can look at user-deposited data detailing how an antibody performed in...Figure 2: Search options in the Antibody Data Hub. Data reports  Each antibody submission is used to create...immunocytochemistry experiment performed with the anti-Lhx6.1 antibody.    For a more detailed understanding of... the antibody performed.  Figure 5: Results for the data report shown in Figure 3. The antibody is rated...requesting an Addgene antibody, the Data Hub can help you decide if an antibody is a good candidate for...

  7. Antibody Validation for Flow Cytometry

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    Blog Post
    ...reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It is also important to seek out ...Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody staining...0.35 µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al...multiple antibody clones targeting the same protein (independent antibodies). If two antibodies, that recognize...Good Antibodies, with significant input from Michael Biddle, PhD, also from Only Good Antibodies. The ...2023). Scaling of an antibody validation procedure enables quantification of antibody performance in major...Antibody validation is to confirm (or refute) that the antibody is selectively detecting the target-of-interest...

  8. Antibodies 101: Fab Fragments

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    Blog Post
    ...regions of the antibody that actually bind antigens.      Figure 1: IgG antibody structure with...portion of the antibody. Fab fragments are useful when you just need an antibody’s antigen binding function...   Why Fabs?  Antibody fragments have a handful of advantages over full antibodies. Their smaller size...as primary antibodies, you can make your own antibody fragments as needed.  In contrast, anti-IgG antibodies...secondary antibody recognizes endogenous antigens. In these cases, even though one full antibody may be ...Prospects of Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028    Wessel GM...However, sometimes you want a tool that is a little more compact — more of an antibody fragment rather than...

  9. Antibodies 101: Flow Compensation

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    Blog Post
    ...your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore record...BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your flow ...

  10. Year of the Camelid: Antibody Style

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    Blog Post
    ...heavy-chain only antibodies in the serum of a camel and soon after found similar antibodies in llamas and...Comparison of a heavy-chain only antibody and single-domain antibody, under the commercial "Nanobody" ...to other antibodies and affinity reagents. But for a quick refresher, some of the advantages of sdAbs ...conventional primary antibodies, and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however...single-domain antibodies genetically fused to fluorescent proteins and used to visualize target antigens in living...single-domain antibodies without the need for a species that naturally produces heavy-chain only antibodies. (A)...heavy chain only antibodies) (B) Nanomice express hybrid heavy-chain only antibodies that are the source...

  11. Antibodies 101 Series on TikTok

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    Blog Post
    ...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ...bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what...what is an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new ...new antibodies animation has all the information! Link in bio. #STEM #antibodies #molecularbiology #science...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...

  12. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...limited understanding of plant biology is a significant technical obstacle to plant synthetic biology. We...pigments (anthocyanins), and the plant circadian clock. Petunias have been a model organism for plant research...here. The first transgenic plant was engineered over 30 years ago, but plant synthetic biology is still...because working with plants can be challenging, there are a lot of unexplored areas in plant biotechnology that...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...pivot towards plant biotechnology and provide a much needed boost to basic molecular plant research, more...the lack of resources associated with plant synthetic biology. Plant molecular biology has traditionally...

  13. Six Spooky Science Stories and Halloween at Addgene

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    Blog Post
    ...science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their ...atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’s body. The fruiting...their behavior. These ants become “zombies” and leave their foraging trail. They climb upwards until they ...temperature and humidity favors fungal growth. The ants bites down and locks their jaws into a leaf, and... onto the forest below for the next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus...genes were appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating ...

  14. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...editor would restore antibiotic resistance. To find such a protein, they created a mutant library of E. coli...improving base editing platforms for plant systems.  Figure 2: Plant base editor platforms and their...components. Adenine base editors for plant We developed plant adenine base editor (ABE) vectors to install... Cytosine base editors for plant There are several CBEs available for plant systems. Target-AID based ... Figure 3: Activity windows of plant base editors. Plant CBE and ABE with expanded PAM compatibility...constructed a number of plant base editors replacing the SpCas9 with the mutant versions of SpCas9 and ...the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID:...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    ...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should ...value greatly. Applying this mantra to AAV selection, there are three important factors related to location...and using AAVs have in common? The three most important items when looking for a house and choosing an...location, location! Just like this common real estate mantra, the choice of serotype (which determines which...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism for...and 9, not only do they undergo retrograde and anterograde transport, but they also have similar transport...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...s blog Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...  Once you get your exciting new antibody, the second thing you’ll want to do is store it away safely. ... check it whenever you use the antibody. This information is important, and you’ll need to track it throughout...accidentally leave your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they...light-sensitive conjugate on the antibody left exposed to light?  Has this antibody been left out, gone through...are planning to conjugate your antibody in the lab, you’ll therefore want to research your conjugation ...

  17. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...license.    In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers...Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies  ... is a cell, but it could be debris or unbound antibodies.) Each event read by the machine is plotted as...conceptualize where the populations are, try drawing a quadrant over the plot that divides it into four, using...different populations by imagining (or drawing) a quadrant over the dot plot. Created with biorender.com....Biorender! While a helpful visualization tool, a quadrant will often be too clean for real-world data. Most...using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/or cellular...

  18. Antibody Data Repositories and Search Engines

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    Blog Post
    ... validated antibody, we recommend using antibody search engines and antibody data repositories to find... that can help: Antibody data repositories, which share validation data, and antibody search engines, ...many different antibody catalogs at the same time.  If you're looking for an antibody, especially a validated...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —...find an antibody for your experiment. Search engines let you easily find and compare available antibodies...and experimental data to help you decide if the antibody is a good fit for your experiment. They come in...

  19. Introducing the Addgene Antibodies 101 eBook!

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    Blog Post
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...101 blog posts on an antibody-specific topic, allowing you to easily access relevant information for planning...re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful...to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...excited to share the very first edition of our Antibodies 101 eBook! The latest addition to our eBook collection...

  20. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection...Addgene blog Antibodies 101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies, targeting... to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies used...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist...fluorophore-conjugated secondary antibody is added that binds the conserved, host species-specific (“anti-rabbit”) region...region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...
Showing: 41 - 60 of 1087 results