...whole ectodomains of cell surface receptors are flanked by two 8 bp cutting enzymes: NotI at the 5' end...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

...helping scientists successfully adopt these tools. Thank you for your contributions and your commitment to...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

...both the Cre and Flp recombinases to remove genes flanked by either loxP or frt sites respectively, and empty...

...single construct would be technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas...

...providing scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

...threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription ...

...phosphosites”) consist of a central phosphoserine site flanked by 15 amino acids on either side occurring within...

... transgenic mice. Contains a loxP-flanked cassette pBTG (pBigT-IRES-GFP) -...

...Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas...

...(Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang...

...-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody (FB)...Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets Optimized...mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets by: ...: Mike Lacy Adding to their set of anti-HA frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi... have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling. Here...variety of applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and...Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies have a variety of applications, including live-cell...

...the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left- and ...BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector...the entry clone containing the DNA of interest flanked by attL sites. As a byproduct of the reaction, ... LR reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression..., an expression clone with the DNA of interest flanked by attB sites is generated. As in the BP reaction... generate our desired entry clone - human KRAS flanked by attL sites.  Method A: recombination of an attB-PCR...create an entry clone: recombination of an attB-flanked PCR product with an attP-containing donor vector...

...summary per batch. Ranking guides for HDR in-depth In other tools, guides are ranked by specificity of ...genome by eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian...employs biological best practices. For example, it ranks guides by CFD score, and it mutates HDR donor templates...between 0 and 1) so that they can be used in the rank equation above. Cut-to-insert distance was fitted...in the graph below) so that it can be used in the rank equation above. Generally, the greater this distance...chanzuckerberg.com. We're eager to hear your thoughts. Many thanks to our guest blogger, Greg Dingle! Greg Dingle ...

...innovation between legal and scientific fields.” Thanks to our legal team, and great customer service, ...and our visibility is increasing internationally, thanks to evolving outreach tactics, increasing business... have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular website in the world...Directors is mature and poised to support our growth. Thanks to Joanna Brownstein (our outgoing Chair) and Dave... incorporate additional Board best practices. I thank them wholeheartedly for their service and support...excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations to current...current and alumni Addgenies on our success. Thanks to our amazing team, we also don’t have to worry about...

...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs. When these plasmids...promoters, the gRNAs are transcribed together and are flanked by specific sites that allow them to be cleaved...nuclease system to cleave the gRNAs. gRNAs are flanked by glycine tRNAs to create polycistronic glycine...CRISPathBrick plasmid contains a nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested...

...in brackets and add the upstream and downstream flanking sequences to their input string (Figure 2). This...provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0 score. For the best...editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves time and resources... Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland),...L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing efficiency...Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime...