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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 499 results for: Ank

Showing: 41 - 60 of 499 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

  2. AAVED

    Type
    Collection
    ...helping scientists successfully adopt these tools. Thank you for your contributions and your commitment to...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...both the Cre and Flp recombinases to remove genes flanked by either loxP or frt sites respectively, and empty...

  5. TALEN Guide

    Type
    Collection
    ...single construct would be technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas...

  6. Viral Vectors

    Type
    Collection
    ...providing scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...

  8. Ras Pathway

    Type
    Collection
    ...threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription ...

  11. The Pleiades Promoter Project

    Type
    Collection
    ...Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...(Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    ...-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody (FB)...Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets Optimized...mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets by: ...: Mike Lacy Adding to their set of anti-HA frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi... have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling. Here...variety of applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and...Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies have a variety of applications, including live-cell...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left- and ...BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector...the entry clone containing the DNA of interest flanked by attL sites. As a byproduct of the reaction, ... LR reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression..., an expression clone with the DNA of interest flanked by attB sites is generated. As in the BP reaction... generate our desired entry clone - human KRAS flanked by attL sites.  Method A: recombination of an attB-PCR...create an entry clone: recombination of an attB-flanked PCR product with an attP-containing donor vector...

  15. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...summary per batch. Ranking guides for HDR in-depth In other tools, guides are ranked by specificity of ...genome by eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian...employs biological best practices. For example, it ranks guides by CFD score, and it mutates HDR donor templates...between 0 and 1) so that they can be used in the rank equation above. Cut-to-insert distance was fitted...in the graph below) so that it can be used in the rank equation above. Generally, the greater this distance...chanzuckerberg.com. We're eager to hear your thoughts. Many thanks to our guest blogger, Greg Dingle! Greg Dingle ...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...innovation between legal and scientific fields.” Thanks to our legal team, and great customer service, ...and our visibility is increasing internationally, thanks to evolving outreach tactics, increasing business... have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular website in the world...Directors is mature and poised to support our growth. Thanks to Joanna Brownstein (our outgoing Chair) and Dave... incorporate additional Board best practices. I thank them wholeheartedly for their service and support...excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations to current...current and alumni Addgenies on our success. Thanks to our amazing team, we also don’t have to worry about...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs. When these plasmids...promoters, the gRNAs are transcribed together and are flanked by specific sites that allow them to be cleaved...nuclease system to cleave the gRNAs. gRNAs are flanked by glycine tRNAs to create polycistronic glycine...CRISPathBrick plasmid contains a nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...in brackets and add the upstream and downstream flanking sequences to their input string (Figure 2). This...provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0 score. For the best...editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves time and resources... Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland),...L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing efficiency...Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...copies the sequence from a repair template with flanking sequence homology for error-free DSB repair. NHEJ...MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA. The DNA ends are chewed back...figure above, short (5-25 bp) regions of homology flanking a double-stranded break yield precise deletion... is likely to introduce additional nucleotides flanking the insertion. If the DNA ends anneal incorrectly...5’ and 3’ microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks...The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA binding...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...reversible. Whereas the excision of a piece of DNA flanked by loxP/FRT sites is favored over its reintroduction...vectors, the DNA sequence that should be inverted is flanked by two pairs of target sites that differ in their...with identical sequence leads to inversion of the flanked DNA sequence and the interior target sites. In ... leaving behind a vector with the inverted DNA flanked by non-compatible target sites (Schnütgen et al...genes in specific cells at specific times. Many thanks to our guest blogger, Katrin Michel! Katrin Michel.... PubMed Central PMCID: PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional Strategy for Monitoring...
Showing: 41 - 60 of 499 results