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We narrowed to 94 results for: Blue fluorescent Protein

Showing: 41 - 60 of 94 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post
    ...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to ... a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction...assemble the full structure of the fluorescent protein, producing fluorescent signal. Image modified from Feng...Multiplexed labeling of cellular proteins with split fluorescent protein tags. Tamura R, Jiang F, Xie J,...Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis... and GFP(11) Versatile protein tagging in cells with split fluorescent protein. Kamiyama et al. Nat Commun...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...VVDfast homodimerizes under blue light iLID-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational...plasma membrane under blue light CRY2-CIB1 heterodimerizes or forms clusters under blue light Check out our... or CIB1 to your protein of interest and express the other separately, then use blue light to generate... implant or a worm wiggling back and forth under blue light — but there’s a lot more to optogenetics than...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...SspB-mCherry-p60 (katanin) are recruited to microtubules under blue light, removing tubulin dimers from the microtubule...license. A naturally occurring microtubule-severing protein, Katanin is of course named for the samurai sword...

  3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ... found that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere...that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence is not essential...fused to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used ...green and red fluorescent proteins) allow simultaneous identification of more than one protein of interest...interfere with fused protein function Under some circumstances, the fused fluorescent proteins can be a roadblock... in the expression of the protein of interest. The green fluorescent protein (GFP) and mCherry, two of...trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins can, indeed, interfere...

  4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post
    ...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent protein to the ...10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular... in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...this vector is suitable for flow cytometry and fluorescence microscopy, they also show that this method ...

  5. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ...where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody binds...suitable antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently conjugated ... palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show...Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence Resources...then you use a microscope to see where the target protein is (localization) and how much there is (quantification...quantification). But what if you’ve got several interesting protein targets and you want to know if they co-localize...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes ... localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae... especially useful in protein complex studies where tagging of multiple protein products is desired, as... unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes...advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe.... A given insert will typically contain both a protein modification sequence and a selectable gene product...several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection...

  7. Antibody Production

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    Collection
    ...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...known concentration and stained with Coomassie blue. Protein band intensities are measured using ImageJ software...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...

  8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... the Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors...other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing...expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret...glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and ...

  9. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors can...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors and ...the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its...optogenetic activators and inhibitors that respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1...the control vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato...

  10. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...tags to your gene, and may have even put a fluorescent protein downstream, separated by an IRES element....galE15 galK16 rpsL(StrR) endA1 λ- XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic...companion post describing protein expression strains, where we go over the basics of protein expression in E. ...general lab strains designed for subcloning or protein expression. If you were to include customized strains...gene Increases the stability of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage...fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed... cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq lac repressor...

  11. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan. The... David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and color...their fusion to a POI can affect protein folding and protein-protein interactions. Also, overexpression...have created antigenic protein tags called “spaghetti monster” fluorescent proteins (smFPs). smFPs are literally...Neuronal tracers such as epitope tags and fluorescent proteins (FP) have also been important in mapping...With the limitations of epitope tags and fluorescent proteins, it has been crucial to develop new tracers...intrinsically fluorescent and can be detected with highly epitope-specific, fluorescently-labeled antibodies...

  12. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post
    ...DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent dyes that fluoresce when...and their corresponding fluorescence. That way, you can compare the fluorescence of your sample against...acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best at 280 nm and organic...sequencing. Unlike absorbance-based methods, fluorescence-based methods require a standard curve, a set...this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis...These fragments are quantified over time using a fluorescent dye that intercalates into the DNA such that ... using capillary electrophoresis which shows fluorescence units over time. Smaller fragments migrate more...

  13. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...the available green fluorescent proteins emit light. The pKa of fluorescent proteins can be accurately ...use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et al.,...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources... Browse the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse...co-factor binding proteins, systems wherein a protein binds a synthetic, fluorescent analogue have been...for another protein. Effectively, it equips the protein of interest with an additional protein module that...Structural Dynamics of Green Fluorescent Protein Fused with a Single Chain Fv Protein." Journal of Biological...

  14. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and pyruvate...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and pyruvate...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...cyan-excitable red fluorescent protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1...on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously...as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...Podcast Segment New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently...recently developed a synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a...evolution of new RFP variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly ...larger strategy to engineer improved red fluorescent proteins (RFPs) and, while the authors note that ...

  16. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...epitopes fused to the protein of interest are recognized and thus fluorescently labeled by scFv antibodies...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists... Upon stimulation with blue light, the complex recruits a CIB1 binding protein (a portion of CRY2) fused...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter... sites for the fluorescent proteins and an inducible promoter (Plasmid 64543or 64542) When the reporter...

  17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair...GO works by affecting protein translation of different reporter proteins. Protein translation requires ...the translation of any detectable protein, not just fluorescent proteins. Figure 4: Reporters in Gene...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...insertion that disrupts fluorescence due to a frameshift. Restoration of fluorescence can only occur through...monitors APOBEC (a family of cytidine deaminase proteins) and Cas9-mediated editing in real time (St. Martin...mCherry expression. Cells can then be sorted using Fluorescence-activated cell sorting (FACS) and the editing...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post
    ...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...transduced by only one RGB AAV and red, green, or blue are the predominate colors expressed (figure 4c)...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

  19. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ... blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent protein...short), where a fluorescent protein is inserted into one of the loop regions of any G-protein-coupled receptor...NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use...calcium indicator using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720) and the Ca2+...engineering of potassium binding proteins, they developed a family of fluorescent potassium indicators with ...requirements (Lu et al. 2022). By fusing two bright fluorescent proteins with different thermal quenching rates (...protein plasmids and resources Fluorescent protein guide: Biosensors Collection ...

  20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ...containing two fluorescent proteins and four repeats of a miRNA target site. One fluorescent protein (EBFP2) ...B (BoNT/B), which cleaves the synaptic protein VAMP2, a protein essential for synaptic transmission. Cleavage...pair of proteins (iLID and SspB) that undergo light-induced dimerization. Upon application of blue light...and function of microbes rely heavily on fluorescent protein genes. These optical reporters have limited...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow... lab discovered in 2014 that these gas vesicle proteins scatter sound waves and create ultrasound contrast...
Showing: 41 - 60 of 94 results