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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,018 results for: CAL;

Showing: 41 - 60 of 1018 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted...present, the virus can spread from this cell to synaptically connected cells (meaning, it can spread to cells...complementation, G-deleted rabies virus can spread to monosynaptically connected cells. To further refine the targeting... was modified so that it could only infect a genetically specified neuronal population. This is done using...leukosis virus. EnvA can direct virus infection specifically into cells that express the TVA viral receptor...EnvA-pseudotyped RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...References Wickersham IR, Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a ...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...differentiate into multiple cell types, but are typically limited to related cell lineages. Pluripotent ...organism. iPSC technology removes the possible ethical concerns related to embryonic stem cell use and...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming...Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015...pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 ...fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol.... Pereira Fibroblasts Neurons Lentiviral Human Chemical modulation of transcriptionally enriched signaling...

  3. Optogenetics Plasmid Collection

    Type
    Collection
    ...be used as an optical switch to control protein interactions, dimerization, localization or activity of...optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate cells and...Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids...the opsin in order to track its expression and localization. Many other types of proteins can also be controlled...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's... Eligible for Viral Packaging PI Return to top Optical Switch Plasmids Browse, sort, or search the table...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters NIC Wiki PSFP : Nikon...Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection Blog: Which FP Should...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life... proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue/UV Cyan... tagging with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - ...Nature Methods July 2016, Vol. 13(7):557-62 Olympus Confocal : The Fluorescent Protein Color Palette NIC Wiki... Shcherbakova et al. : Journal of the American Chemical Society, May 2012, Vol. 134 No.18, pp. 7913–7923...

  5. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein...Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator... Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your...domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor...Insert Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify ...Selectable Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... site into a genomic attP site. So far, their practical application has been limited by low integration...sequential strand exchange at a specific target site (called loxP for Cre, or FRT for Flp). The nature of the...other recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre...repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC...Expression: A stop codon flanked with loxP sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream...Placing the loxP sites on either side of a gene (called "floxing", for "flanked by loxP"), will permit ...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

  7. Chemogenetics Plasmids

    Type
    Collection
    ...) domains that respond specifically to small molecules termed Pharmacologically Selective Effector Molecules.... Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are activated by specific... to artificially activate or inhibit specific biological processes. Addgene’s collection contains two ...Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...have been engineered to respond to specific physiologically inert small molecules. There are four types...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ..., excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Fenno LE, Ramakrishnan C, Kim YS, Deisseroth K, Callaway EM, Oberhuber M, Conzelmann KK, Staiger JF. 2019...Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

  9. Genetic Code Expansion

    Type
    Collection
    ...incorporate a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which...aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid containing the ...the gene of interest with the modified codon (typically the amber codon) that is recognized by the cognate...plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated using the existing...acid onto the tRNA. The tRNA and synthetase are called an orthogonal set, because they should not crosstalk...the tRNA with a different amino acid. They are typically expressed from a single plasmid, with multiple...to specific stimuli, such as light. On a broader scale, the expanded genetic code can help us understand...

  10. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...to help identify guide sequences that minimize identical genomic matches or near-matches to reduce risk...band” is often too large to efficiently amplify. Typically use primers at least 100 bp from the predicted... to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary. Run...inoculate into a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep...clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter. Primer Validation...cells) before screening individual clones. It is critical that the deletion and non-deletion amplicons be...Masuda T, Maeda T, Paw BH, Orkin SH. Journal of Biological Chemistry . 2014. PubMed . Correction of a genetic...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...’s disease (PD) is a chronic and progressive neurological disorder that primarily affects movement. More...-wide association studies (GWAS) or other large scale studies. Researchers are now investigating the role...indicators with improved activation kinetics and localization for imaging synaptic transmission. Aggarwal ... transcription factors for sensory, motor, and cortical neuron differentiation. Held et al. Cell Rep. ...fluorescent proteins, including Markers of Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids...(Link opens in a new window) A collection of neurological mouse models including complex and monogenic...

  12. Plan Your Experiment

    Type
    Collection
    ...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when ...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...from a single cell and will result in cells with identical genomes. If your end goal is to establish an edited...Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens... Kampmann, M., & Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ..., which allows you to visualize the cellular localization of your protein. Additionally, tags are...expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without...pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector...vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector... the plasmid. Selectable Marker Typical Host Organism Representative Empty ...

  14. Promega Plasmid Collection

    Type
    Collection
    ...monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in...HaloTags form a covalent bond with these synthetic chemical ligands, which have been developed for special...levels in lysates or live-cells, thus preserving physiological relevance. NanoBiT Assays NanoLuc Binary Technology...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

  15. AAV Packaged on Request

    Type
    Collection
    ...choice of seven serotypes and four volumes. Details Scale and Titer Packaged on Request can be ordered in ...preparations, we set both minimum standards and typical titers, though actual yields may vary by construct...construct. Size Quantity Total Volume Minimum Titer Typical Titer Small NEW 2 × 100 µL aliquots 0.2 mL 4 × ... with a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see...providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution....

  16. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...for Brain Science BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Packaged on Request AAV Guide...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https:...

  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein...Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator...Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify ...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...assessment of lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard...

  19. Lentivirus Plasmids

    Type
    Collection
    ...divided among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table lists common...target cells. This genome is encoded by plasmids called "transfer plasmids," which can be modified to encode...

  20. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...to mentors, virtual and local professional development, membership to a local hub of educators teaching...Hypotheses in a Malate Dehydrogenase Oriented CURE. Callahan KP et al. CourseSource. 2022 Jan 14. DOI: 10.24918...Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74...
Showing: 41 - 60 of 1018 results