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We narrowed to 92 results for: Cre recombinase

Showing: 41 - 60 of 92 results

  1. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed...infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP... a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer... 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...Express iCre under from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and ...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji Iwasato...pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer...-inducible expression of a light-activated Cre recombinase. Jacco van Rheenen 104102–104112 TetBow Series...Repressible Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids...tetracycline-controlled transactivator (tTA) was created by fusing TetR with the activation domain of the...tetracycline-controlled transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline...

  3. Brain Armamentarium

    Type
    Collection
    ...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction...But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained ...marker, likely by breeding to a mouse line with a recombinase. If it turned out your chimeric mice did not ...plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA construct as ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...repair template is used. The original design for creating a conditional allele, or floxed mouse, from Yang... and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-...

  5. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...genetic tools: activators, inhibitors, sensors, recombinases, and more. With the help of adeno-associated...Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common approaches... strategies Combining different approaches can increase the specificity of cell targeting even further...delivery of AAV at the injection site results in increased specificity of the targeted cell population.  ...are also developing ways to improve delivery by increasing transgene cargo capacity, enhancing anterograde...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-... constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-...membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein...Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV ...

  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...extracted from a sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially...contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence...based detection of SARS-CoV-2 genetic material (CREST) Sensitivity: 10 copies of RNA per microliter Time...

  8. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions...community members. In addition we’ve experienced incredible growth with our readership expanding from ~10,000...next revolution in biological research. We’re incredibly happy to see that one of our career posts has...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...open-reading frame (DIO). Upon coexpression with cre recombinase (either from a mouse line or viral co-injection...specificity dependent on the promoter of the cre recombinase while still taking advantage of the robust ...an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer required...tracing with fluorescent proteins in nontransgenic and cre driver mice. Current protocols in neuroscience / ...: PMC4242517.  7. Madisen L, et al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced... us to show causality in neural circuits with incredible temporal and spatial precision (I’m not going...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...

  10. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603...mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5...

  11. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...made with BioRender.   Grow plasmids in recombinase-deficient strains Since plasmid multimerization...plasmid in a bacterial strain that is deficient in recombinase protein RecA can reduce the frequency of multimerization...generated by SnapGene software.  Thanks to increasing affordability and accessibility, more researchers...by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies to isolate the monomer Sometimes... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...DNA-forming sequences and WRN deficiency independently increase the frequency of base substitution in human cells... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

  12. AAV Viral Preps

    Type
    Collection
    ...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...will only be expressed in cells that express cre recombinase. Typically, this feature is used to attain ...Expression of hM4D(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD...cell-specific expression in transgenic animals, where cre expression is restricted to a particular cell type...the first time, the Roth Lab recommends verifying cre expression prior to use. The lab additionally recommends...expressing cell lines, which can then be used both for screens (using pooled CRISPR knockout libraries) and individual...Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then...monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D...

  14. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...excised by intentional introduction of the Cre recombinase. This is a time-tested system but does require...control when you turn off expression for good, the Cre-Lox system offers inducible yet permanent gene excision...some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for ...!  Turn it on Turn it on forever Permanently increasing or turning on expression of a new protein is ... gRNA, to your gene of interest to temporarily increase expression. There are several flavors of activators...

  15. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    .... In tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...the Esr1 domain that blocks Cre expression. Then, Cre is expressed and Cre-mediated recombination of floxed...in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of DNA sequences...that regulates Cre expression. The most common drug used to induce the expression of Cre is tamoxifen. ...doxycycline mediated activation of Cre to either induce or repress Cre expression using TetON or TetOFF ...using Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox ... vector, creating the floxed gene segment. Crossing of mice containing a floxed gene to a Cre-expressing...

  16. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... combines prime editing with a site-specific recombinase to achieve insertion of long sequences. In their...genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular...

  17. Retrovirus Plasmids

    Type
    Collection
    ...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...overexpression plasmid. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the ...largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells. See the Weinberg Lab page...pBABE-hygro-hTERT MoMLV Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin...cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE... post-transcriptional regulatory element that increases export to the cytoplasm. William Hahn 11375 MDH1...

  19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce modifications...light or noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider... also to minimize the damage of tissue due to increased pressure of the added liquid. The volume should...editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be observed after...

  20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...floxed dCas9 so that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9...T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing...are dioxygenases that oxidize 5-methylcytosine to create rare DNA bases that are recognized by distinct ...
Showing: 41 - 60 of 92 results