We narrowed to 103 results for: EXO

1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   Blog Post

   ...implant Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled...

2. FastCloning: A Newer, Simpler Cloning Technique

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   Blog Post

   ...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...

3. Plan Your Experiment

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   Collection

   ...regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces ... expression of a truncated protein. Targeting an exon coding for an essential protein domain could abolish...

4. What the HEK?

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   Blog Post

   ...Membrane receptors and ion channels are often exogenously expressed and studied in HEKs due to the cell...

5. Advice for Scientists Starting a Lab

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   Blog Post

   ...Professor who was always “off flying his plane to exotic places while occasionally emailing us his worldly...

6. Viral Vectors 101: Biosensors

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   Blog Post

   ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

7. GCE4All: Making Genetic Code Expansion Accessible

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   Blog Post

   ...because it uses ∆serB strains, does not require exogenously added phosphoserine. A community resource Like...

8. Viral Vectors 101: Viral Applications

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   Blog Post

   ...specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for example. Some customization...

9. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ... it may be possible to direct insertion of an exogenous DNA fragment at a Cas9 targeted break (or pair...

10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

    ...pathogens. Clift et al. found that addition of exogenous TRIM21 protein along with an antibody targeting...

11. Human Germline Editing Using CRISPR

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    Blog Post

    ... editing sites in the embryos, as well as whole exome sequencing of embryo derived-ES cells.They also ...

12. CRISPRainbow and Genome Visualization

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    Blog Post

    ...labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific and sensitive...

13. What's New in CRISPR - November 2021

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    Blog Post

    ...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

14. All in a Twist: dsRNA

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    Blog Post

    ...with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...co-infected into the cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond...

16. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20...

17. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ... made of 3 types of proteins: fiber, penton and hexon proteins. The viral genome DNA is further protected...

18. Making CRISPR Plasmids Using Fragmid

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    Blog Post

    ...destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    .... Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version...

20. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ...