We narrowed to 103 results for: EXO

1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

   Type

   Blog Post

   ...immunoblotting to detect. Another approach is to target two exonic sites within a single gene using paired DSBs. This...

2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   ...implant Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled...

3. FastCloning: A Newer, Simpler Cloning Technique

   Type

   Blog Post

   ...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...

4. What the HEK?

   Type

   Blog Post

   ...Membrane receptors and ion channels are often exogenously expressed and studied in HEKs due to the cell...

5. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...Professor who was always “off flying his plane to exotic places while occasionally emailing us his worldly...

6. Viral Vectors 101: Biosensors

   Type

   Blog Post

   ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

7. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   ...because it uses ∆serB strains, does not require exogenously added phosphoserine. A community resource Like...

8. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   ...specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for example. Some customization...

9. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   ... it may be possible to direct insertion of an exogenous DNA fragment at a Cas9 targeted break (or pair...

10. All in a Twist: dsRNA

    Type

    Blog Post

    ...with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA...

11. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...co-infected into the cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond...

13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...pathogens. Clift et al. found that addition of exogenous TRIM21 protein along with an antibody targeting...

14. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ... editing sites in the embryos, as well as whole exome sequencing of embryo derived-ES cells.They also ...

15. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific and sensitive...

16. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    ...a few advantages over other cloning methods. Exonuclease-based methods like Gibson assembly require 20...

17. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short...

18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    .... Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version...

19. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ... made of 3 types of proteins: fiber, penton and hexon proteins. The viral genome DNA is further protected...

20. Of Myc and Men

    Type

    Blog Post

    ... wanted to distinguish between endogenous and exogenous versions of the same target. In the 1980s, Munro...